NOVEL METHOD OF DETECTION OF BIREFRINGENT OCULAR AMYLOIDOSIS DEPOSITS USING MONOCHROMATIC WAVELENGTHS OF SCANNING LASER OPHTHALMOSCOPY.
| Title: | NOVEL METHOD OF DETECTION OF BIREFRINGENT OCULAR AMYLOIDOSIS DEPOSITS USING MONOCHROMATIC WAVELENGTHS OF SCANNING LASER OPHTHALMOSCOPY. |
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| Authors: | Zafar S; Retina Service, Wills Eye Hospital and Mid Atlantic Retina, Philadelphia, Pennsylvania.; August A; Retina Service, Wills Eye Hospital and Mid Atlantic Retina, Philadelphia, Pennsylvania.; Lin S; National Institute of Health Research Biomedical Research Centre at Moorfields Eye Hospital and the UCL Institute of Ophthalmology, London, United Kingdom.; Division of Evolution, Infection and Genomics, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, United Kingdom.; Manchester Centre for Genomic Medicine, Department of Ophthalmology, Saint Mary's Hospital and Manchester Royal Eye Hospital, Manchester University NHS Foundation Trust, Manchester, United Kingdom.; Acaba-Berrocal L; Retina Service, Wills Eye Hospital and Mid Atlantic Retina, Philadelphia, Pennsylvania.; Beca F; Retina Service, Wills Eye Hospital and Mid Atlantic Retina, Philadelphia, Pennsylvania.; Bowe T; Retina Service, Wills Eye Hospital and Mid Atlantic Retina, Philadelphia, Pennsylvania.; Patel SN; Retina Service, Wills Eye Hospital and Mid Atlantic Retina, Philadelphia, Pennsylvania.; Yonekawa Y; Retina Service, Wills Eye Hospital and Mid Atlantic Retina, Philadelphia, Pennsylvania.; Vander J; Retina Service, Wills Eye Hospital and Mid Atlantic Retina, Philadelphia, Pennsylvania.; Mahroo OA; National Institute of Health Research Biomedical Research Centre at Moorfields Eye Hospital and the UCL Institute of Ophthalmology, London, United Kingdom.; UCL Institute of Ophthalmology, London, United Kingdom.; UCL Institute of Ophthalmology, University College, London, United Kingdom.; Keane PA; National Institute of Health Research Biomedical Research Centre at Moorfields Eye Hospital and the UCL Institute of Ophthalmology, London, United Kingdom.; UCL Institute of Ophthalmology, London, United Kingdom.; UCL Institute of Ophthalmology, University College, London, United Kingdom.; Fertala A; Department of Orthopedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania.; Pavesio C; Uveitis and Scleritis Service, Moorfields Eye Hospital Foundation Trust, London, United Kingdom.; Petrushkin H; Uveitis and Scleritis Service, Moorfields Eye Hospital Foundation Trust, London, United Kingdom.; Milman T; Department of Pathology, Wills Eye Hospital, Thomas Jefferson University, Philadelphia, Pennsylvania; and.; Pulido JS; Retina Service, Wills Eye Hospital and Mid Atlantic Retina, Philadelphia, Pennsylvania.; Department of Translational Ophthalmology, Wills Eye Hospital, Philadelphia, Pennsylvania. |
| Corporate Authors: | Moorfields Amyloid Group |
| Source: | Retina (Philadelphia, Pa.) [Retina] 2026 Jan 01; Vol. 46 (1), pp. 156-162. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 8309919 Publication Model: Print Cited Medium: Internet ISSN: 1539-2864 (Electronic) Linking ISSN: 0275004X NLM ISO Abbreviation: Retina Subsets: MEDLINE |
| Imprint Name(s): | Publication: Hagerstown, MD : Lippincott Williams & Wilkins; Original Publication: Philadelphia : Lippincott, [1981?- |
| MeSH Terms: | Amyloid Neuropathies, Familial*/diagnosis ; Ophthalmoscopy*/methods; Humans ; Retrospective Studies ; Male ; Female ; Aged ; Middle Aged ; Case-Control Studies ; Birefringence ; Aged, 80 and over ; Sensitivity and Specificity ; Adult ; Fundus Oculi |
| Abstract: | Purpose: To describe a noninvasive imaging technique for the detection of ocular amyloidosis using ultra-widefield images.; Methods: This was a retrospective, case-control study of patients with familial transthyretin amyloidosis seen at the Wills Eye Hospital and Moorfields Eye Hospital, United Kingdom. Pseudocolor, 100% red, and 100% green ultra-widefield fundus images of patients with familial transthyretin were reviewed, and s ensitivity, specificity, and area under the curve for the pattern abnormalities noted on fundus images for amyloidosis were calculated.; Results: A total of 19 eyes of 10 patients with transthyretin amyloidosis were included. Vitreous floaters were the most common posterior segment manifestation present in 57.9% (N = 11/19) eyes. The sensitivity and specificity for a hypointense or isointense pattern on 100% red and a hyperintense pattern on 100% green being amyloidosis were 57.9% (95% CI: 33.5%‒79.7%) and 100% (95% CI: 54.1%‒100%), respectively. The area under the curve was 0.79 (95% CI: 0.68-0.90). Excluding the hypomorphic V122I mutation, the sensitivity and specificity for a hypointense or isointense pattern on 100% red and a hyperintense pattern on 100% green being amyloidosis were 64.7% (95% CI: 38.3%‒85.8%) and 100% (54.1%‒100%), respectively. The area under the curve was 0.82 (95% CI: 0.71-0.94). Histopathological analysis showed that amyloid deposits were visible under green and blue filters but not under red filters.; Conclusion: The appearance of amyloid deposits can vary based on the type of scanning laser used. The methodology described in this study represents a novel and noninvasive approach to detect ocular amyloidosis with relatively high specificity. |
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| Grant Information: | McNamara Retina Research Fund |
| Contributed Indexing: | Keywords: Optos; amyloid; imaging; ultra-widefield |
| SCR Disease Name: | Amyloidosis, Hereditary, Transthyretin-Related |
| Entry Date(s): | Date Created: 20250902 Date Completed: 20251218 Latest Revision: 20260130 |
| Update Code: | 20260131 |
| DOI: | 10.1097/IAE.0000000000004648 |
| PMID: | 40893117 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't