Effects of the cryotop vitrification method for the cryopreservation of in vitro produced Cỏ goat embryos.
| Title: | Effects of the cryotop vitrification method for the cryopreservation of in vitro produced Cỏ goat embryos. |
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| Authors: | Nguyen VK; Key Laboratory of Animal Cell Biotechnology, National Institute of Animal Sciences, Hanoi, Vietnam. Electronic address: cotihin@gmail.com.; Vu HTT; Key Laboratory of Animal Cell Biotechnology, National Institute of Animal Sciences, Hanoi, Vietnam.; Hoang AT; Key Laboratory of Animal Cell Biotechnology, National Institute of Animal Sciences, Hanoi, Vietnam.; Pham YTK; Key Laboratory of Animal Cell Biotechnology, National Institute of Animal Sciences, Hanoi, Vietnam.; LE DV; Key Laboratory of Animal Cell Biotechnology, National Institute of Animal Sciences, Hanoi, Vietnam.; Nguyen LAT; Key Laboratory of Animal Cell Biotechnology, National Institute of Animal Sciences, Hanoi, Vietnam.; Nhien NT; Faculty of Biotechnology, Vietnam National University of Agriculture, Hanoi, Vietnam.; Van Anh NT; Faculty of Biomedical Science, Phenikaa University, Hanoi, Vietnam.; Pham LD; Key Laboratory of Animal Cell Biotechnology, National Institute of Animal Sciences, Hanoi, Vietnam. |
| Source: | Cryobiology [Cryobiology] 2025 Dec; Vol. 121, pp. 105323. Date of Electronic Publication: 2025 Sep 13. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0006252 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2392 (Electronic) Linking ISSN: 00112240 NLM ISO Abbreviation: Cryobiology Subsets: MEDLINE |
| Imprint Name(s): | Publication: : Amsterdam : Elsevier; Original Publication: San Diego, CA : Academic Press |
| MeSH Terms: | Cryopreservation*/methods ; Cryopreservation*/veterinary ; Goats*/embryology ; Cryoprotective Agents*/pharmacology ; Blastocyst*/cytology ; Blastocyst*/drug effects ; Blastocyst*/physiology ; Vitrification*; Fertilization in Vitro/veterinary ; Dimethyl Sulfoxide/pharmacology ; Zygote/cytology ; Zygote/drug effects ; Ethylene Glycol/pharmacology ; Animals ; Female ; Embryo, Mammalian ; Embryonic Development |
| Abstract: | This study investigated the influence of embryonic developmental stage, cryoprotectant concentration, and vitrification solution volume on the cryopreservation efficiency of in vitro-produced Cỏ goat embryos. In Experiment 1 the survival rate of zygotes cryopreserved 20 h after IVF was lower than blastocysts cryopreserved 7 days after IVF (66.86 % vs 90.92 %, P |
| Competing Interests: | Conflict of interest The authors declare no conflicts of interest. |
| Contributed Indexing: | Keywords: CPA concentration; Embryo development stage; IVP Cỏ goat embryos; Vitrification; Vitrification solution volume |
| Substance Nomenclature: | 0 (Cryoprotective Agents); YOW8V9698H (Dimethyl Sulfoxide); FC72KVT52F (Ethylene Glycol) |
| Entry Date(s): | Date Created: 20250914 Date Completed: 20251127 Latest Revision: 20251127 |
| Update Code: | 20260130 |
| DOI: | 10.1016/j.cryobiol.2025.105323 |
| PMID: | 40946385 |
| Database: | MEDLINE |
Journal Article