Integration of Point-of-Care Technology in the Decoding Process of Single Nucleotide Polymorphism for Healthcare Application †.
| Title: | Integration of Point-of-Care Technology in the Decoding Process of Single Nucleotide Polymorphism for Healthcare Application †. |
|---|---|
| Authors: | Trinh TND; Biotechnology Institute, Tra Vinh University, Vinh Long 98000, Vietnam.; Nguyen HA; Department of Molecular Biology, Institute of Food and Biotechnology, Can Tho University, Can Tho 95000, Vietnam.; Thi NPA; Department of Molecular Biology, Institute of Food and Biotechnology, Can Tho University, Can Tho 95000, Vietnam.; Ho TXT; Institute for Global Health Innovations, Duy Tan University, Da Nang 58000, Vietnam.; Trinh KTL; Advanced Materials Technology Institute, Vietnam National University Ho Chi Minh City, Ho Chi Minh City 70000, Vietnam.; Vietnam National University, Ho Chi Minh City 70000, Vietnam.; Tran NKS; NTT Hi-Tech Institute, Nguyen Tat Thanh University, District 09, Ho Chi Minh City 70000, Vietnam.; Nguyen Tat Thanh University Center for Hi-Tech Development, Saigon Hi-Tech Park, Ho Chi Minh City 70000, Vietnam. |
| Source: | Micromachines [Micromachines (Basel)] 2025 Oct 13; Vol. 16 (10). Date of Electronic Publication: 2025 Oct 13. |
| Publication Type: | Journal Article; Review |
| Language: | English |
| Journal Info: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101640903 Publication Model: Electronic Cited Medium: Print ISSN: 2072-666X (Print) Linking ISSN: 2072666X NLM ISO Abbreviation: Micromachines (Basel) Subsets: PubMed not MEDLINE |
| Imprint Name(s): | Original Publication: Basel, Switzerland : MDPI, [2010]- |
| Abstract: | Single nucleotide polymorphism (SNP) involves plenty of genetic disorders in organisms that can be passed down to the next generation or cause the stimulant signal that leads to early mortality in infants, especially within humankind. In medical field, real-time polymerase chain reaction (RT-PCR) is the most popular method for disease diagnosis. The investigation of genetic maps for the prediction of inherited illnesses needs the collaboration of sequencing technique and genome analysis. Although these methods are popular now, the cost for each test is quite high. Moreover, there is the requirement of extra machines and skillful technician or specialist level. Among these popular methods, the allele-specific polymerase chain reaction (AS-PCR), allele-specific loop isothermal mediated amplification (AS-LAMP), and allele-specific recombinase polymerase amplification (AS-RPA) are brought up for screening the nucleotide differences in the genetic sequence which will be noticed in this review as their availability, novelty, and potential for quick distinguishing of disease caused by SNP. Point-of-care testing (POCT) is a system built in a portable size but can perform the entire process of SNP recognition. Along with that, the POCT is intersected with the mentioned amplification methods and the genetic material preparation steps to become a united framework for higher efficiency and accuracy and lower cost. According to that, this review will focus on three common amplification techniques and their combination with POCT in the upstream and downstream process to genotype SNP related to human diseases. |
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| Contributed Indexing: | Keywords: loop-mediated isothermal amplification; point-of-care testing; polymerase chain reaction; recombinase polymerase amplification; single nucleotide polymorphism |
| Entry Date(s): | Date Created: 20251029 Date Completed: 20251029 Latest Revision: 20251101 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC12566449 |
| DOI: | 10.3390/mi16101159 |
| PMID: | 41156406 |
| Database: | MEDLINE |
Journal Article; Review