Prevalence, molecular characterization, and therapeutic management of Buxtonella sulcata in buffaloes from Western Uttar Pradesh, India.
| Title: | Prevalence, molecular characterization, and therapeutic management of Buxtonella sulcata in buffaloes from Western Uttar Pradesh, India. |
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| Authors: | Maurya PS; Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut 250110, India.; Ramakant; Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya 224229, India.; Singh A; Department of Veterinary Medicine, College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut 250110, India.; Jaiswal V; Department of Veterinary Pathology, College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut 250110, India.; Sarkar TK; Department of Veterinary Medicine, College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut 250110, India.; Pal A; Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut 250110, India.; Upadhyay S; Department of Animal Biotechnology, College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut 250110, India.; Verma AK; Department of Veterinary Medicine, College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut 250110, India. Electronic address: drakverma79@gmail.com. |
| Source: | Veterinary parasitology, regional studies and reports [Vet Parasitol Reg Stud Reports] 2025 Oct; Vol. 65, pp. 101342. Date of Electronic Publication: 2025 Sep 10. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101680410 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2405-9390 (Electronic) Linking ISSN: 24059390 NLM ISO Abbreviation: Vet Parasitol Reg Stud Reports Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam : Elsevier, [2015]- |
| MeSH Terms: | Buffaloes*/parasitology ; Ciliophora Infections*/veterinary ; Ciliophora Infections*/epidemiology ; Ciliophora Infections*/drug therapy ; Ciliophora Infections*/parasitology ; Paramphistomatidae*/genetics ; Paramphistomatidae*/isolation & purification; India/epidemiology ; Feces/parasitology ; RNA, Ribosomal, 18S/genetics ; Polymerase Chain Reaction/veterinary ; Animals ; Prevalence ; Phylogeny |
| Abstract: | Buxtonella sulcata is an opportunistic protozoan parasite commonly affecting ruminants, with significant infection rates reported in cattle and buffaloes worldwide. This large cyst-forming ciliate shares morphological similarities with Balantidium coli, a known parasite of humans and pigs. In the present study, 512 fecal samples were collected from diarrheic and non-diarrheic buffaloes across five districts Meerut, Saharanpur, Muzaffarnagar, Baghpat, and Hapur in Western Uttar Pradesh, India, between April 2023 and March 2024. Samples were subjected to both microscopic examination and polymerase chain reaction (PCR) targeting the 18S rRNA gene, followed by sequencing and phylogenetic analysis. The overall prevalence of B. sulcata was found to be 33.59 % by microscopy and 35.16 % by PCR. Confirmed cases were treated with halquinol bolus administered orally and long-acting oxytetracycline administered intramuscularly. This study highlights the notable presence of B. sulcata in the buffalo population of Western Uttar Pradesh and emphasizes the importance of molecular diagnostics for accurate detection. Furthermore, the research supports the efficacy of a combination therapeutic regimen and recommends its strategic application to manage buxtonellosis in affected regions.; (Copyright © 2025 Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that there are no conflicts of interest related to this study. |
| Contributed Indexing: | Keywords: Buffaloes; Buxtonella sulcata; Diarrhoea; Genetic evaluation; India; PCR |
| Substance Nomenclature: | 0 (RNA, Ribosomal, 18S) |
| Entry Date(s): | Date Created: 20251116 Date Completed: 20251116 Latest Revision: 20251116 |
| Update Code: | 20260130 |
| DOI: | 10.1016/j.vprsr.2025.101342 |
| PMID: | 41242798 |
| Database: | MEDLINE |
Journal Article