Extracellular Vesicles Derived From Antral Follicles Significantly Change the Transcriptional Profile of Cumulus Cells and Oocytes During Pre-In Vitro Maturation in Cattle.
| Title: | Extracellular Vesicles Derived From Antral Follicles Significantly Change the Transcriptional Profile of Cumulus Cells and Oocytes During Pre-In Vitro Maturation in Cattle. |
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| Authors: | Mingoti GZ; School of Veterinary Medicine, Laboratory of Reproductive Physiology, Universidade Estadual Paulista, Araçatuba, Sao Paulo, Brazil.; Graduate Program in Veterinary Medicine, School of Agrarian and Veterinary Sciences, Universidade Estadual Paulista, Jaboticabal, Sao Paulo, Brazil.; Nunes GB; Graduate Program in Veterinary Medicine, School of Agrarian and Veterinary Sciences, Universidade Estadual Paulista, Jaboticabal, Sao Paulo, Brazil.; Souza-Cáceres MB; Departament of Genetics and Evolution, Center for Biological and Health Sciences, Federal University of Sao Carlos, Sao Carlos, Sao Paulo, Brazil.; da Silva CR; Graduate Program in Veterinary Medicine, School of Agrarian and Veterinary Sciences, Universidade Estadual Paulista, Jaboticabal, Sao Paulo, Brazil.; Nociti RP; Department of Veterinary Medicine, University of Sao Paulo, Pirassununga, Sao Paulo, Brazil.; de Athayde FF; School of Veterinary Medicine, Laboratory of Reproductive Physiology, Universidade Estadual Paulista, Araçatuba, Sao Paulo, Brazil.; Mogollón-García HD; Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, Universidade Estadual Paulista, Botucatu, Sao Paulo, Brazil.; Bastos NM; Department of Veterinary Medicine, University of Sao Paulo, Pirassununga, Sao Paulo, Brazil.; Rosa PMS; Department of Veterinary Medicine, University of Sao Paulo, Pirassununga, Sao Paulo, Brazil.; de Oliveira Alves L; Departament of Genetics and Evolution, Center for Biological and Health Sciences, Federal University of Sao Carlos, Sao Carlos, Sao Paulo, Brazil.; Pereira-Júnior SAG; Departament of Genetics and Evolution, Center for Biological and Health Sciences, Federal University of Sao Carlos, Sao Carlos, Sao Paulo, Brazil.; Franchi FF; Graduate Program in Pharmacology and Biotechnology, Institute of Biosciences, Universidade Estadual Paulista, Botucatu, Sao Paulo, Brazil.; Ferreira JCP; Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, Universidade Estadual Paulista, Botucatu, Sao Paulo, Brazil.; da Silveira JC; Department of Veterinary Medicine, University of Sao Paulo, Pirassununga, Sao Paulo, Brazil.; Chiaratti MR; Departament of Genetics and Evolution, Center for Biological and Health Sciences, Federal University of Sao Carlos, Sao Carlos, Sao Paulo, Brazil. |
| Source: | Molecular reproduction and development [Mol Reprod Dev] 2025 Nov; Vol. 92 (11), pp. e70068. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Wiley-Liss Country of Publication: United States NLM ID: 8903333 Publication Model: Print Cited Medium: Internet ISSN: 1098-2795 (Electronic) Linking ISSN: 1040452X NLM ISO Abbreviation: Mol Reprod Dev Subsets: MEDLINE |
| Imprint Name(s): | Publication: : Hoboken, N.J. : Wiley-Liss; Original Publication: New York, NY : A.R. Liss, 1988- |
| MeSH Terms: | Cumulus Cells*/metabolism ; Cumulus Cells*/cytology ; Extracellular Vesicles*/metabolism ; Oocytes*/metabolism ; Oocytes*/cytology ; In Vitro Oocyte Maturation Techniques*/methods ; Ovarian Follicle*/metabolism ; Ovarian Follicle*/cytology ; Transcriptome*; Animals ; Cattle ; Female |
| Abstract: | Cumulus-oocyte complexes (COCs) used for in vitro production (IVP) of bovine embryos originate from antral follicles of different sizes, leading to variations in developmental competence. To address this, pre-in vitro maturation (pre-IVM) allows oocytes with additional time to acquire developmental competence. Given the role of follicular fluid-derived extracellular vesicles (EVs) in ovarian follicle communication, which has been shown to vary in content and function across folliculogenesis, we investigated whether EVs from early versus late antral follicles influence COCs during pre-IVM. EV supplementation significantly altered gene expression in cumulus cells and oocytes. In cumulus cells, affected pathways included MAPK signaling, Gap junctions, Cytokine-cytokine receptor interaction, Axon guidance, cAMP, and Cushing syndrome. In oocytes, fewer genes were altered, with effects on Inositol phosphate metabolism, p53 signaling and Cholesterol metabolism. Despite these changes, no significant effects of the EV treatment were noted on oocyte chromatin configuration and developmental competence, except for a significant increase of mitochondrial membrane potential (Δψm) in blastocysts. In conclusion, EV supplementation during pre-IVM significantly altered the transcriptional profile of COCs, with EVs from early follicles modulating the expression of genes regulating cumulus cell proliferation and gap junctions, while EVs from late follicles impacted pathways associated with meiotic resumption, cumulus cell expansion, and apoptosis. Along with improved Δψm in blastocysts, these results support a positive effect of EVs on bovine COCs, but further research is needed to better characterize the functional consequences, mainly in terms of the effects of early versus late follicle-derived EVs on oocyte developmental potential.; (© 2025 The Author(s). Molecular Reproduction and Development published by Wiley Periodicals LLC.) |
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| Grant Information: | This work was supported by CAPES (Finance Code 001), CNPq (313505/2021-7 and 305544/2025-0) and FAPESP (12/18297-7, 19/11174-6, 20/15412-6, 21/09886-8, 21/06645-0, 21/14019-1 and 23/04558-8). |
| Contributed Indexing: | Keywords: RNA‐Seq; bovine; extracellular vesicles; follicle development; mitochondria; oocyte competence; pre‐IVM |
| Entry Date(s): | Date Created: 20251125 Date Completed: 20251125 Latest Revision: 20260512 |
| Update Code: | 20260513 |
| PubMed Central ID: | PMC12645189 |
| DOI: | 10.1002/mrd.70068 |
| PMID: | 41287464 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't