Genomic and Biological Insights of Bacteriophage ΦBc24 Targeting Bacillus cereus.
| Title: | Genomic and Biological Insights of Bacteriophage ΦBc24 Targeting Bacillus cereus. |
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| Authors: | Tran NK; Department of Molecular Microbiology, Institute of Biology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi 100000, Vietnam.; Department of Life Sciences, University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi 100000, Vietnam.; Lanh PT; Department of Molecular Microbiology, Institute of Biology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi 100000, Vietnam.; Trinh Thu T; Department of Molecular Microbiology, Institute of Biology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi 100000, Vietnam.; Phuoc MH; Department of Molecular Microbiology, Institute of Biology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi 100000, Vietnam.; Duy ND; Department of Molecular Microbiology, Institute of Biology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi 100000, Vietnam.; Hien VT; Department of Molecular Microbiology, Institute of Biology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi 100000, Vietnam.; Quyen DV; Department of Molecular Microbiology, Institute of Biology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi 100000, Vietnam.; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi 100000, Vietnam. |
| Source: | Current issues in molecular biology [Curr Issues Mol Biol] 2025 Oct 31; Vol. 47 (11). Date of Electronic Publication: 2025 Oct 31. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 100931761 Publication Model: Electronic Cited Medium: Internet ISSN: 1467-3045 (Electronic) Linking ISSN: 14673037 NLM ISO Abbreviation: Curr Issues Mol Biol Subsets: PubMed not MEDLINE |
| Imprint Name(s): | Publication: 2021- : Basel, Switzerland : MDPI; Original Publication: Wymondham, Norfolk, UK : Caister Academic Press |
| Abstract: | Foodborne illnesses associated with Bacillus cereus represent a persistent public health concern. In this study, we described the isolation and characterization of a novel bacteriophage, ΦBc24, from mud samples, which showed lytic activity against foodborne pathogen B. cereus. Transmission electron microscopy revealed that ΦBc24 exhibited a myovirus morphotype. Biological assays demonstrated that its narrow host range was restricted to B. cereus strains and efficient lytic activity, characterized by a latent period of 10 min and a burst size of 40 PFU per infected cell. The phage exhibited high physicochemical stability, tolerating pH values of 2-12, temperatures of 4-50 °C, salinity up to 1 M NaCl, and ultraviolet exposure, while effectively suppressing host bacterial growth for up to six hours. Whole-genome sequencing showed that phage ΦBc24 possessed a double-stranded DNA genome of 160,311 bp, with 39.48% GC content, and 269 predicted open reading frames (ORFs). Remarkably, 11 tRNA genes were identified, whereas no genes associated with lysogeny, virulence, or antimicrobial resistance were detected. Phylogenetic analysis suggested that ΦBc24 belongs to the genus Caeruleovirus, subfamily Bastillevirinae, family Herelleviridae. Taken together, these results highlight the biological robustness and genomic safety of ΦBc24, supporting its potential as a biocontrol candidate against the foodborne pathogen B. cereus. |
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| Grant Information: | Grant No. NVQG 2022/ĐT.06 Ministry of Science and Technology |
| Contributed Indexing: | Keywords: Bastillevirinae; Caeruleovirus; Herelleviridae; biocontrol; food contamination; phage therapy |
| Entry Date(s): | Date Created: 20251126 Date Completed: 20251126 Latest Revision: 20251128 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC12651245 |
| DOI: | 10.3390/cimb47110906 |
| PMID: | 41296410 |
| Database: | MEDLINE |
Journal Article