Chemically Anchored Diamond with H3 Centers for Ratiometric Measurement of Isolated Mitochondria Temperature.
| Title: | Chemically Anchored Diamond with H3 Centers for Ratiometric Measurement of Isolated Mitochondria Temperature. |
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| Authors: | Romshin AM; Prokhorov General Physics Institute of the Russian Academy of Sciences, 119991 Moscow, Russia.; Kruglov AG; Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, 142290 Pushchino, Russia.; Nikiforova AB; Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, 142290 Pushchino, Russia.; Zhivopistsev AA; Prokhorov General Physics Institute of the Russian Academy of Sciences, 119991 Moscow, Russia.; Bagramov RH; Vereshchagin Institute of High Pressure Physics of the Russian Academy of Sciences, 108840 Moscow, Russia.; Korepanov VI; Institute of Microelectronics Technology and High-Purity Materials of the Russian Academy of Sciences, 142432 Chernogolovka, Russia.; Pasternak DG; Prokhorov General Physics Institute of the Russian Academy of Sciences, 119991 Moscow, Russia.; Shlyapnikov YM; Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, 142290 Pushchino, Russia.; Valitov TM; Prokhorov General Physics Institute of the Russian Academy of Sciences, 119991 Moscow, Russia.; Physics Faculty, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia.; Filonenko VP; Vereshchagin Institute of High Pressure Physics of the Russian Academy of Sciences, 108840 Moscow, Russia.; Vlasov II; Prokhorov General Physics Institute of the Russian Academy of Sciences, 119991 Moscow, Russia. |
| Source: | International journal of molecular sciences [Int J Mol Sci] 2025 Nov 25; Vol. 26 (23). Date of Electronic Publication: 2025 Nov 25. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Basel, Switzerland : MDPI, [2000- |
| MeSH Terms: | Diamond*/chemistry ; Mitochondria, Liver*/metabolism ; Mitochondria, Liver*/physiology ; Mitochondria*; Animals ; Rats ; Temperature ; Oxidative Phosphorylation |
| Abstract: | Precise measurement of mitochondrial temperature at different metabolic states remains one of the key challenges in cellular biophysics due to the lack of thermometers that combine nanoscale sensitivity with stable thermal contact with the organelle. Here, we present a hybrid sensing platform based on chemically functionalized diamond microparticles containing H3 luminescent centers, covalently bound to the outer membrane of isolated rat liver mitochondria. Surface activation via oxidation and EDC/HOBt chemistry provides a robust and reproducible thermal link between the thermometric probe and the organelle, minimizing heat dissipation through the surrounding medium. The local temperature is monitored ratiometrically from the emission ratio of H3 centers at 515-525 nm and 585-610 nm, showing a linear dependence on temperature with a relative sensitivity of 1.15%⋅°C-1 in aqueous environments. Upon the uncoupling of oxidative phosphorylation and the inhibition of electron transport, the diamond thermometers reproducibly recorded the local thermal changes in the range of 0.5-10 °C, depending on the degree of coverage by anchored mitochondria. The observed response reflects efficient local heat confinement within the diamond-mitochondrion assembly, suggesting that structural organization and thermal insulation at the subcellular level are critical modulators of mitochondrial thermogenesis. |
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| Grant Information: | 23-14-00129 Russian Science Foundation |
| Contributed Indexing: | Keywords: cell thermodynamics; color centers; diamond thermometer; mitochondria |
| Substance Nomenclature: | 7782-40-3 (Diamond) |
| Entry Date(s): | Date Created: 20251211 Date Completed: 20251211 Latest Revision: 20251214 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC12692260 |
| DOI: | 10.3390/ijms262311395 |
| PMID: | 41373552 |
| Database: | MEDLINE |
Journal Article