Antimicrobial Functionalized Mesoporous Silica FDU-12 Loaded with Bacitracin.
| Title: | Antimicrobial Functionalized Mesoporous Silica FDU-12 Loaded with Bacitracin. |
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| Authors: | Vasile DA; Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Chemical Engineering and Biotechnologies, National University of Science and Technology POLITEHNICA Bucharest, Gh. Polizu 1-7, 011061 Bucharest, Romania.; National Research Center for Food Safety, National University of Science and Technology POLITEHNICA Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania.; National Center for Micro and Nanomaterials, National University of Science and Technology POLITEHNICA Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania.; Motelica L; National Research Center for Food Safety, National University of Science and Technology POLITEHNICA Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania.; National Center for Micro and Nanomaterials, National University of Science and Technology POLITEHNICA Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania.; Advanced Research Center for Innovative Materials, Products and Processes, National University of Science and Technology POLITEHNICA Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania.; Mîrț LA; Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Chemical Engineering and Biotechnologies, National University of Science and Technology POLITEHNICA Bucharest, Gh. Polizu 1-7, 011061 Bucharest, Romania.; National Research Center for Food Safety, National University of Science and Technology POLITEHNICA Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania.; National Center for Micro and Nanomaterials, National University of Science and Technology POLITEHNICA Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania.; National Institute for Research & Development in Chemistry and Petrochemistry-ICECHIM, Spl. Independentei 202, 060021 Bucharest, Romania.; Vasilievici G; National Institute for Research & Development in Chemistry and Petrochemistry-ICECHIM, Spl. Independentei 202, 060021 Bucharest, Romania.; Memecică OM; Department of Bioresources and Polymer Sciences, Faculty of Chemical Engineering and Biotechnologies, National University of Science and Technology POLITEHNICA Bucharest, Gh. Polizu 1-7, 011061 Bucharest, Romania.; Oprea OC; National Research Center for Food Safety, National University of Science and Technology POLITEHNICA Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania.; National Center for Micro and Nanomaterials, National University of Science and Technology POLITEHNICA Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania.; Department of Inorganic Chemistry, Physical Chemistry and Electrochemistry, Faculty of Chemical Engineering and Biotechnologies, National University of Science and Technology POLITEHNICA Bucharest, Gh. Polizu 1-7, 011061 Bucharest, Romania.; Academy of Romanian Scientists, Ilfov Street 3, 050044 Bucharest, Romania.; Surdu AV; Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Chemical Engineering and Biotechnologies, National University of Science and Technology POLITEHNICA Bucharest, Gh. Polizu 1-7, 011061 Bucharest, Romania.; National Research Center for Food Safety, National University of Science and Technology POLITEHNICA Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania.; National Center for Micro and Nanomaterials, National University of Science and Technology POLITEHNICA Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania.; Department of Materials Science, Faculty of Materials Science and Engineering, Transilvania University of Brasov, 29 Eroilor Blvd., 500036 Brasov, Romania.; Trușcă RD; National Research Center for Food Safety, National University of Science and Technology POLITEHNICA Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania.; National Center for Micro and Nanomaterials, National University of Science and Technology POLITEHNICA Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania.; Chircov C; Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Chemical Engineering and Biotechnologies, National University of Science and Technology POLITEHNICA Bucharest, Gh. Polizu 1-7, 011061 Bucharest, Romania.; National Research Center for Food Safety, National University of Science and Technology POLITEHNICA Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania.; National Center for Micro and Nanomaterials, National University of Science and Technology POLITEHNICA Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania.; Vasile BȘ; National Research Center for Food Safety, National University of Science and Technology POLITEHNICA Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania.; National Center for Micro and Nanomaterials, National University of Science and Technology POLITEHNICA Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania.; Advanced Research Center for Innovative Materials, Products and Processes, National University of Science and Technology POLITEHNICA Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania.; Ghizdavet ZD; Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Chemical Engineering and Biotechnologies, National University of Science and Technology POLITEHNICA Bucharest, Gh. Polizu 1-7, 011061 Bucharest, Romania.; National Research Center for Food Safety, National University of Science and Technology POLITEHNICA Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania.; National Center for Micro and Nanomaterials, National University of Science and Technology POLITEHNICA Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania.; Ficai D; Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Chemical Engineering and Biotechnologies, National University of Science and Technology POLITEHNICA Bucharest, Gh. Polizu 1-7, 011061 Bucharest, Romania.; National Research Center for Food Safety, National University of Science and Technology POLITEHNICA Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania.; National Center for Micro and Nanomaterials, National University of Science and Technology POLITEHNICA Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania.; Department of Inorganic Chemistry, Physical Chemistry and Electrochemistry, Faculty of Chemical Engineering and Biotechnologies, National University of Science and Technology POLITEHNICA Bucharest, Gh. Polizu 1-7, 011061 Bucharest, Romania.; Academy of Romanian Scientists, Ilfov Street 3, 050044 Bucharest, Romania.; Albu AM; Department of Bioresources and Polymer Sciences, Faculty of Chemical Engineering and Biotechnologies, National University of Science and Technology POLITEHNICA Bucharest, Gh. Polizu 1-7, 011061 Bucharest, Romania.; Pericleanu R; Department of Botany and Microbiology, Faculty of Biology, University of Bucharest, Intr. Portocalelor No. 1-3, 060101 Bucharest, Romania.; Dumbravă AȘ; Department of Botany and Microbiology, Faculty of Biology, University of Bucharest, Intr. Portocalelor No. 1-3, 060101 Bucharest, Romania.; Mihai MM; Department of Oncologic Dermatology, 'Elias' Emergency University Hospital, 'Carol Davila' University of Medicine and Pharmacy, 020021 Bucharest, Romania.; The Research Institute of the University of Bucharest (ICUB), B.P Hasdeu No. 7, 050095 Bucharest, Romania.; Gheorghe-Barbu I; Department of Botany and Microbiology, Faculty of Biology, University of Bucharest, Intr. Portocalelor No. 1-3, 060101 Bucharest, Romania.; The Research Institute of the University of Bucharest (ICUB), B.P Hasdeu No. 7, 050095 Bucharest, Romania.; Ficai A; Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Chemical Engineering and Biotechnologies, National University of Science and Technology POLITEHNICA Bucharest, Gh. Polizu 1-7, 011061 Bucharest, Romania.; National Research Center for Food Safety, National University of Science and Technology POLITEHNICA Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania.; National Center for Micro and Nanomaterials, National University of Science and Technology POLITEHNICA Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania.; Academy of Romanian Scientists, Ilfov Street 3, 050044 Bucharest, Romania. |
| Source: | Molecules (Basel, Switzerland) [Molecules] 2026 Jan 19; Vol. 31 (2). Date of Electronic Publication: 2026 Jan 19. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Basel, Switzerland : MDPI, c1995- |
| MeSH Terms: | Silicon Dioxide*/chemistry ; Bacitracin*/pharmacology ; Bacitracin*/chemistry ; Anti-Bacterial Agents*/pharmacology ; Anti-Bacterial Agents*/chemistry ; Drug Carriers*/chemistry ; Anti-Infective Agents*/pharmacology ; Anti-Infective Agents*/chemistry; Biofilms/drug effects ; Porosity ; Drug Liberation ; Microbial Sensitivity Tests ; Drug Delivery Systems ; Humans |
| Abstract: | The threats leading to the extinction of humanity accelerate the evolution and development of materials that are capable of providing conditions for preserving health and, implicitly, life. In our work, we developed drug delivery systems based on mesoporous silica which can deliver an antibiotic, bacitracin, in a more controlled manner. The synthesis of the FDU-12 was performed through a sol-gel method and alternatively functionalized with -NH2 groups or with poly(N-acryloylmorpholine) chains. The loading of bacitracin was performed using the vacuum-assisted method we successfully used to load these mesoporous materials preferentially within the pores as proved by the TGA-DSC results. The release was performed in two types of simulated body fluid (SBF) and this process was evaluated with chromatographic method using UV detection. The obtained data were fitted in three mathematical models of kinetic drug release (Weibull model, Korsmeyer-Peppas model, and nonlinear regression). The antimicrobial evaluation demonstrated that bacitracin-loaded FDU-12 formulations exhibited strong activity against both reference and clinical Staphylococcus strains. At sub-inhibitory concentrations, all formulations significantly reduced microbial adherence and biofilm formation, although certain strain-dependent stimulatory effects were observed. Furthermore, exposure to sub-MIC levels modulated the production of soluble virulence factors (hemolysins, lipase, and amylase), in a formulation- and strain-dependent manner, underscoring the ability of surface-functionalized FDU-12 carriers to influence bacterial pathogenicity while enhancing antimicrobial efficacy. |
| References: | PLoS One. 2021 Jan 22;16(1):e0245583. (PMID: 33481897); Microorganisms. 2023 Sep 28;11(10):. (PMID: 37894097); Int J Biol Macromol. 2012 May 1;50(4):1048-54. (PMID: 22421216); Acta Pol Pharm. 2010 May-Jun;67(3):217-23. (PMID: 20524422); Signal Transduct Target Ther. 2023 Nov 24;8(1):435. (PMID: 37996406); Materials (Basel). 2022 Nov 11;15(22):. (PMID: 36431468); Sci Rep. 2018 Jan 17;8(1):989. (PMID: 29343865); Biomater Sci. 2022 Jun 14;10(12):3268-3281. (PMID: 35583113); Molecules. 2015 Oct 30;20(11):19690-8. (PMID: 26528964); J Am Chem Soc. 2008 Jul 16;130(28):9006-12. (PMID: 18570415); Trop Med Health. 2023 Jun 7;51(1):33. (PMID: 37287083); Inorg Chem. 2020 Jul 20;59(14):10275-10284. (PMID: 32628466); Molecules. 2022 Jun 30;27(13):. (PMID: 35807477); Mol Microbiol. 2017 May;104(3):365-376. (PMID: 28142193); PLOS Glob Public Health. 2024 Apr 16;4(4):e0003077. (PMID: 38626068); Int J Biol Macromol. 2024 Oct;277(Pt 4):134567. (PMID: 39116970); Int J Pharm X. 2019 Feb 22;1:100008. (PMID: 31517273); Carbohydr Polym. 2012 Oct 1;90(2):784-91. (PMID: 22840002); J Phys Chem B. 2006 Nov 2;110(43):21467-72. (PMID: 17064096); J Colloid Interface Sci. 2017 Jul 1;497:217-225. (PMID: 28285049); Int J Pharm Investig. 2015 Jul-Sep;5(3):124-33. (PMID: 26258053); Drug Deliv. 2021 Dec;28(1):856-864. (PMID: 33928831); J Crit Care. 2002 Sep;17(3):188-202. (PMID: 12297996); Emerg Infect Dis. 2011 Oct;17(10):1917-20. (PMID: 22000371); Pathogens. 2023 Apr 17;12(4):. (PMID: 37111495); Langmuir. 2007 Apr 10;23(8):4315-23. (PMID: 17348693); Phys Chem Chem Phys. 2015 Jun 7;17(21):13882-94. (PMID: 25946487); Saudi Pharm J. 2018 Dec;26(8):1089-1097. (PMID: 30532629); Molecules. 2021 Nov 27;26(23):. (PMID: 34885775); Antibiotics (Basel). 2019 Apr 11;8(2):. (PMID: 30979069); Angew Chem Int Ed Engl. 2008;47(28):5163-6. (PMID: 18528828); Int J Med Microbiol. 2014 Mar;304(2):103-9. (PMID: 24439196); Nanomaterials (Basel). 2020 Apr 25;10(5):. (PMID: 32344938); J Fungi (Basel). 2024 Aug 09;10(8):. (PMID: 39194889); Int J Pharm. 2014 Mar 25;463(2):170-6. (PMID: 23871740); Des Monomers Polym. 2017 Aug 23;20(1):468-475. (PMID: 29491818); Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7297-301. (PMID: 7638184); Front Biosci. 2002 Aug 01;7:d1825-42. (PMID: 12133812); BMC Infect Dis. 2024 Sep 28;24(1):1065. (PMID: 39342123); Sci Rep. 2023 Apr 27;13(1):6873. (PMID: 37105997); Molecules. 2022 Aug 30;27(17):. (PMID: 36080354); Microbiol Spectr. 2018 Feb;7(2):. (PMID: 30953424); Angew Chem Int Ed Engl. 2003 Jul 14;42(27):3146-50. (PMID: 12866103); Antimicrob Agents Chemother. 1992 May;36(5):955-61. (PMID: 1510419); Pharmaceutics. 2023 Feb 16;15(2):. (PMID: 36839982); Microbiol Spectr. 2019 Mar;7(2):. (PMID: 30927347); Nanomaterials (Basel). 2022 May 12;12(10):. (PMID: 35630870); Assay Drug Dev Technol. 2021 May-Jun;19(4):226-236. (PMID: 33891509); Materials (Basel). 2018 Dec 20;12(1):. (PMID: 30577550); Langmuir. 2021 Nov 16;37(45):13379-13389. (PMID: 34637312); Clin Microbiol Rev. 2017 Oct;30(4):887-917. (PMID: 28724662); Eur J Pharm Biopharm. 2024 Aug;201:114349. (PMID: 38848782); Int J Mol Sci. 2025 Apr 09;26(8):. (PMID: 40331997); Int J Nanomedicine. 2021 Jun 30;16:4409-4430. (PMID: 34234434); Antibiotics (Basel). 2025 May 06;14(5):. (PMID: 40426537); Int J Mol Sci. 2012 Oct 23;13(10):13726-36. (PMID: 23202977); Pharmaceutics. 2022 Jul 25;14(8):. (PMID: 35893798); ACS Appl Mater Interfaces. 2017 Mar 22;9(11):9470-9483. (PMID: 28252278); Science. 1945 Oct 12;102(2650):376-7. (PMID: 17770204); Int J Mol Sci. 2020 Nov 15;21(22):. (PMID: 33203098); Polymers (Basel). 2021 Aug 27;13(17):. (PMID: 34502937); J Mater Chem B. 2015 Mar 14;3(10):2056-2066. (PMID: 32262373) |
| Grant Information: | 29ROMD/20.05.2024 Unitatea Executiva Pentru Finantarea Invatamantului Superior a Cercetarii Dezvoltarii si Inovarii |
| Contributed Indexing: | Keywords: Staphylococcus spp.; anti-biofilm virulence factors modulation; antimicrobial; bacitracin; drug delivery; kinetic model; mesoporous silica; poly(N-acryloylmorpholine); skin infection; surface functionalization; vacuum-assisted loading |
| Substance Nomenclature: | 7631-86-9 (Silicon Dioxide); 1405-87-4 (Bacitracin); 0 (Anti-Bacterial Agents); 0 (Drug Carriers); 0 (Anti-Infective Agents) |
| Entry Date(s): | Date Created: 20260128 Date Completed: 20260128 Latest Revision: 20260131 |
| Update Code: | 20260131 |
| PubMed Central ID: | PMC12844483 |
| DOI: | 10.3390/molecules31020340 |
| PMID: | 41599388 |
| Database: | MEDLINE |
Journal Article