Search for Molecular Markers of Myocardial Injury in a Mouse Model of Obstructive Nephropathy Using Nestin Promoter-Driven GFP Expression.
| Title: | Search for Molecular Markers of Myocardial Injury in a Mouse Model of Obstructive Nephropathy Using Nestin Promoter-Driven GFP Expression. |
|---|---|
| Authors: | Sokolov IA; A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.; Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.; Zorova LD; A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.; National Medical Research Center of Obstetrics, Gynecology, and Perinatology Named after Academician V. I. Kulakov, Ministry of Health of the Russian Federation, Moscow, Russia.; Zorov DB; A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.; National Medical Research Center of Obstetrics, Gynecology, and Perinatology Named after Academician V. I. Kulakov, Ministry of Health of the Russian Federation, Moscow, Russia.; Plotnikov EY; A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.; National Medical Research Center of Obstetrics, Gynecology, and Perinatology Named after Academician V. I. Kulakov, Ministry of Health of the Russian Federation, Moscow, Russia.; Abramicheva PA; A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia. abramicheva.polina@gmail.com. |
| Source: | Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2025 Nov; Vol. 180 (1), pp. 48-52. Date of Electronic Publication: 2026 Feb 09. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Springer Country of Publication: United States NLM ID: 0372557 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-8221 (Electronic) Linking ISSN: 00074888 NLM ISO Abbreviation: Bull Exp Biol Med Subsets: MEDLINE |
| Imprint Name(s): | Publication: New York : Springer; Original Publication: New York, Consultants Bureau. |
| MeSH Terms: | Nestin*/genetics ; Nestin*/metabolism ; Myocardium*/metabolism ; Myocardium*/pathology ; Ureteral Obstruction*/metabolism ; Ureteral Obstruction*/pathology ; Ureteral Obstruction*/genetics ; Ureteral Obstruction*/complications; Connexin 43/metabolism ; Connexin 43/genetics ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; Biomarkers/metabolism ; WT1 Proteins/metabolism ; WT1 Proteins/genetics ; Natriuretic Peptide, Brain/metabolism ; Natriuretic Peptide, Brain/genetics ; Myosin Heavy Chains/metabolism ; Myosin Heavy Chains/genetics ; Animals ; Mice ; Disease Models, Animal ; Promoter Regions, Genetic ; Male |
| Abstract: | The expression of cardiac dysfunction markers and the number of nestin-positive cells in the myocardium were analyzed in a mouse model of obstructive nephropathy using animals expressing GFP under the nestin promoter. Unilateral ureteral obstruction (UUO) led to an increase in the number of nestin-positive cells in cardiac tissue, accompanied by elevated expression of B-type natriuretic peptide, which positively correlated with GFP levels. A trend toward reduced expression of β-myosin heavy chain and the gap junction protein connexin-43 was observed, whereas the expression of the progenitor cell marker WT1 remained unchanged under UUO conditions. These findings indicate the development of cardiac dysfunction in this model is secondary to primary renal injury and highlight a concomitant expansion of nestin-positive cells within the myocardium.; (© 2026. Springer Science+Business Media, LLC, part of Springer Nature.) |
| References: | Junho CVC, Trentin-Sonoda M, Panico K, et al. Cardiorenal syndrome: long road between kidney and heart. Heart Fail Rev. 2022;27(6):2137-53. https://doi.org/10.1007/s10741-022-10218-w. (PMID: 10.1007/s10741-022-10218-w35133552); Buliga-Finis ON, Ouatu A, Badescu MC, et al. Beyond the Cardiorenal syndrome: pathophysiological approaches and biomarkers for renal and cardiac crosstalk. Diagnostics (Basel). 2022;12(4):773. https://doi.org/10.3390/diagnostics12040773. (PMID: 10.3390/diagnostics12040773354538219028970); Whelan RS, Kaplinskiy V, Kitsis RN. Cell death in the pathogenesis of heart disease: mechanisms and significance. Annu Rev Physiol. 2010;72:19-44. https://doi.org/10.1146/annurev.physiol.010908.163111. (PMID: 10.1146/annurev.physiol.010908.16311120148665); Beltrami AP, Barlucchi L, Torella D, et al. Adult cardiac stem cells are multipotent and support myocardial regeneration. Cell. 2003;114(6):763-76. https://doi.org/10.1016/s0092-8674(03)00687-1. (PMID: 10.1016/s0092-8674(03)00687-114505575); Amini H, Rezaie J, Vosoughi A, et al. Cardiac progenitor cells application in cardiovascular disease. J Cardiovasc Thorac Res. 2017;9(3):127-32. https://doi.org/10.15171/jcvtr.2017.22.; Béguin PC, Gosselin H, Mamarbachi M, et al. Nestin expression is lost in ventricular fibroblasts during postnatal development of the rat heart and re-expressed in scar myofibroblasts. J Cell Physiol. 2012;227(2):813-20. https://doi.org/10.1002/jcp.22794. (PMID: 10.1002/jcp.2279421503881); Buyan MI, Andrianova NV, Popkov VA, et al. Ageassociated loss in renal nestin-positive progenitor cells. Int J Mol Sci. 2022;23(19):11015. https://doi.org/10.3390/ijms231911015. (PMID: 10.3390/ijms231911015362323269569966); Mokrý J, Cízková D, Filip S, et al. Nestin expression by newly formed human blood vessels. Stem Cells Dev. 2004;13(6):658-64. https://doi.org/10.1089/scd.2004.13.658. (PMID: 10.1089/scd.2004.13.65815684833); van Ham WB, Cornelissen CM, van Veen TAB. Uremic toxins in chronic kidney disease highlight a fundamental gap in understanding their detrimental effects on cardiac electrophysiology and arrhythmogenesis. Acta Physiol (Oxf). 2022;236(3):e13888. https://doi.org/10.1111/apha.13888. (PMID: 10.1111/apha.13888361486049787632); Abramicheva PA, Semenovich DS, Zorova LD, et al. Decreased renal expression of PAQR5 is associated with the absence of a nephroprotective effect of progesterone in a rat UUO model. Sci Rep. 2023;13(1):12871. https://doi.org/10.1038/s41598-023-39848-2. (PMID: 10.1038/s41598-023-39848-23755336910409855); Cizkova D, Zurmanova JM, Gerykova L, et al. Nestin expression in intact and hypertrophic myocardium of spontaneously hypertensive rats during aging. J Muscle Res Cell Motil. 2024;45(2):41-51. https://doi.org/10.1007/s10974-023-09641-9. (PMID: 10.1007/s10974-023-09641-936690826); Berry SE, Andruszkiewicz P, Chun JL, et al. Nestin expression in end-stage disease in dystrophindeficient heart: implications for regeneration from endogenous cardiac stem cells. Stem Cells Transl Med. 2013;2(11):848-61. https://doi.org/10.5966/sctm.2012-0174. (PMID: 10.5966/sctm.2012-0174240687413808200); El-Helou V, Dupuis J, Proulx C, et al. Resident nestin+ neural-like cells and fibers are detected in normal and damaged rat myocardium. Hypertension. 2005;46(5):1219-25. https://doi.org/10.1161/01.HYP.0000187888.39665.d9. (PMID: 10.1161/01.HYP.0000187888.39665.d916230517); Tamura Y, Matsumura K, Sano M, et al. Neural crest-derived stem cells migrate and differentiate into cardiomyocytes after myocardial infarction. Arterioscler Thromb Vasc Biol. 2011;31(3):582-9. https://doi.org/10.1161/ATVBAHA.110.214726. (PMID: 10.1161/ATVBAHA.110.21472621212399); Goetze JP, Christoffersen C, Perko M, et al. Increased cardiac BNP expression associated with myocardial ischemia. FASEB J. 2003;17(9):1105-7. https://doi.org/10.1096/fj.02-0796fje. (PMID: 10.1096/fj.02-0796fje12709407); Azevedo JC, Reis BC, Barreto NM, et al. BNP was associated with ischemic myocardial scintigraphy and death in patients at chest pain unit. Arq Bras Cardiol. 2015;104(1):16-23. https://doi.org/10.5935/abc.20140175. (PMID: 10.5935/abc.2014017525409879); Pandya K, Smithies O. β-MyHC and cardiac hypertrophy: size does matter. Circ. Res. 2011;109(6):609-10. https://doi.org/10.1161/CIRCRESAHA.111.252619. (PMID: 10.1161/CIRCRESAHA.111.25261921885833); Verheule S, Kaese S. Connexin diversity in the heart: insights from transgenic mouse models. Front Pharmacol. 2013;4:81. https://doi.org/10.3389/fphar.2013.00081. (PMID: 10.3389/fphar.2013.00081238188813694209); Wagner N, Wagner KD. Every beat you take – The Wilms’ tumor suppressor WT1 and the heart. Int J Mol Sci. 2021;22(14):7675. https://doi.org/10.3390/ijms22147675. (PMID: 10.3390/ijms22147675342992958306835) |
| Contributed Indexing: | Keywords: myocardium; nestin-positive cells; obstructive nephropathy; renocardiac syndrome |
| Substance Nomenclature: | 0 (Nestin); 0 (Connexin 43); 147336-22-9 (Green Fluorescent Proteins); 0 (Biomarkers); 0 (WT1 Proteins); 114471-18-0 (Natriuretic Peptide, Brain); EC 3.6.4.1 (Myosin Heavy Chains); 0 (Nes protein, mouse); 0 (WT1 protein, mouse); 0 (GJA1 protein, mouse) |
| Entry Date(s): | Date Created: 20260209 Date Completed: 20260307 Latest Revision: 20260307 |
| Update Code: | 20260308 |
| DOI: | 10.1007/s10517-026-06578-0 |
| PMID: | 41661429 |
| Database: | MEDLINE |
Journal Article