Growth promotion of milk-associated Candida zeylanoides by a cyclic lipopeptide produced by Pseudomonas.
| Title: | Growth promotion of milk-associated Candida zeylanoides by a cyclic lipopeptide produced by Pseudomonas. |
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| Authors: | Wasmuth I; Department of Paleobiotechnology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute, 07745 Jena, Germany.; Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich Schiller University Jena, 07743 Jena, Germany.; Ibrahim A; Department of Paleobiotechnology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute, 07745 Jena, Germany.; Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich Schiller University Jena, 07743 Jena, Germany.; Köhler P; Institute of Inorganic and Analytical Chemistry, Friedrich Schiller University Jena, 07743 Jena, Germany.; Hovhannisyan H; Institute for Research in Biomedicine, 08028 Barcelona, Spain.; Barcelona Supercomputing Center, 08034 Barcelona, Spain.; CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III, 28029 Madrid, Spain.; Marcet-Houben M; Institute for Research in Biomedicine, 08028 Barcelona, Spain.; Barcelona Supercomputing Center, 08034 Barcelona, Spain.; CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III, 28029 Madrid, Spain.; Gabaldón T; Institute for Research in Biomedicine, 08028 Barcelona, Spain.; Barcelona Supercomputing Center, 08034 Barcelona, Spain.; Catalan Institution for Research and Advanced Studies (ICREA), 08010 Barcelona, Spain.; CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III, 28029 Madrid, Spain.; Tsolmon S; Tanalab, School of Management, Mongolian University of Science and Technology, 13381 Ulaanbaatar, Mongolia.; Warinner C; Department of Paleobiotechnology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute, 07745 Jena, Germany.; Department of Archaeogenetics, Max Planck Institute for Evolutionary Anthropology, 04103 Leipzig, Germany.; Associated Research Group of Archaeogenetics, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute, 07745 Jena, Germany.; Faculty of Biological Sciences, Institute of Microbiology, Friedrich Schiller University Jena, 07743 Jena, Germany.; Department of Anthropology, Harvard University, Cambridge, MA 02138, United States.; Cluster of Excellence Balance of the Microverse, Friedrich Schiller University Jena, 07743 Jena, Germany.; Stallforth P; Department of Paleobiotechnology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute, 07745 Jena, Germany.; Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich Schiller University Jena, 07743 Jena, Germany.; Cluster of Excellence Balance of the Microverse, Friedrich Schiller University Jena, 07743 Jena, Germany. |
| Source: | The ISME journal [ISME J] 2026 Jan 14; Vol. 20 (1). |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Oxford University Press Country of Publication: England NLM ID: 101301086 Publication Model: Print Cited Medium: Internet ISSN: 1751-7370 (Electronic) Linking ISSN: 17517362 NLM ISO Abbreviation: ISME J Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2024- : Oxford : Oxford University Press; Original Publication: London : Nature Pub. Group |
| MeSH Terms: | Pseudomonas*/metabolism ; Pseudomonas*/isolation & purification ; Pseudomonas*/genetics ; Lipopeptides*/metabolism ; Lipopeptides*/chemistry ; Lipopeptides*/pharmacology ; Lipopeptides*/isolation & purification ; Peptides, Cyclic*/metabolism ; Peptides, Cyclic*/chemistry ; Peptides, Cyclic*/pharmacology ; Peptides, Cyclic*/isolation & purification ; Milk*/microbiology ; Candida*/growth & development ; Candida*/drug effects; RNA, Ribosomal, 16S/genetics ; Animals ; Fermentation ; Microbial Interactions ; Magnetic Resonance Spectroscopy |
| Abstract: | Milk has a rich microbiota that engages in complex interactions. These interactions can be mutualistic or antagonistic, shaping the microbial composition of dairy products and affecting fermentation, preservation, and product quality. In this study, we investigate the interaction between Pseudomonas bacteria and the yeast Candida zeylanoides in the context of traditional dairy fermentation. We identified a collection of cyclic lipopeptides (CLPs) from Pseudomonas sp. SM4, isolated from the traditional Mongolian dairy product öröm and elucidated the structure of six CLPs belonging to the amphisin family using a combination of bioinformatic predictions, mass spectrometry, Marfey's analysis, nuclear magnetic resonance (NMR) spectroscopy, and X-ray crystallography. Milk-based bioassays and 16S rRNA gene amplicon sequencing analyses revealed changes in the microbial composition of milk due to the addition of the CLP tensin A and allowed us to identify its function in promoting the growth of C. zeylanoides, a yeast commonly found in dairy environments. Through transcriptomic analysis, we obtained preliminary insights into the potential metabolic pathways that may contribute to the growth-promoting effect, which indicates a role for the glyoxylate metabolism. This represents the first report of a CLP directly stimulating yeast growth. Understanding how Pseudomonas-derived lipopeptides modulate microbial communities, particularly by supporting yeasts relevant to dairy fermentations, could inform strategies for optimizing dairy fermentation processes like kefir production and improving the stability and quality of fermented dairy products.; (© The Author(s) 2026. Published by Oxford University Press on behalf of the International Society for Microbial Ecology.) |
| Grant Information: | CIBERINFEC CB21/13/00061-ISCIII-SGEFI/ERDF Instituto de Salud Carlos III; IMP/00019 Instituto de Salud Carlos III; LCF/PR/HR21/00737 La Caixa; GBMF9742 Gordon and Betty Moore Foundation; SGR01551 Catalan Research Agency; PDC2022-133266-I00 Spanish Ministry of Science and Innovation; PLEC2023-010225 Spanish Ministry of Science and Innovation; PCI2022-135066-2 Spanish Ministry of Science and Innovation; CPP2021-008552 Spanish Ministry of Science and Innovation; PID2021-126067NB-I00 Spanish Ministry of Science and Innovation; Mongolian Dietetic Association; EXC 2051 #390713860 Deutsche Forschungsgemeinschaft; ERC-2018-StG-804884 International ERC_ European Research Council; Werner Siemens Stiftung |
| Contributed Indexing: | Keywords: Candida zeylanoides; Mongolia; cyclic lipopeptides; microbiome; milk |
| Substance Nomenclature: | 0 (Lipopeptides); 0 (Peptides, Cyclic); 0 (RNA, Ribosomal, 16S) |
| Entry Date(s): | Date Created: 20260219 Date Completed: 20260312 Latest Revision: 20260313 |
| Update Code: | 20260313 |
| PubMed Central ID: | PMC12978640 |
| DOI: | 10.1093/ismejo/wrag033 |
| PMID: | 41714189 |
| Database: | MEDLINE |
Journal Article