Identification and Ultrastructural Peculiarities of Nestin-Carrying Progenitor Cells in Kidney.
| Title: | Identification and Ultrastructural Peculiarities of Nestin-Carrying Progenitor Cells in Kidney. |
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| Authors: | Vays VB; A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia.; Vangeli IM; A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia.; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, 117997 Moscow, Russia.; Bakeeva LE; A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia.; Makievskaya CI; Faculty of Bioengineering and Bioinformatics, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia.; Popkov VA; A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia.; Zorova LD; A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia.; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, 117997 Moscow, Russia.; Kireev II; A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia.; Zorov SD; A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia.; Faculty of Bioengineering and Bioinformatics, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia.; Andrianova NV; A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia.; Buyan MI; A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia.; Faculty of Bioengineering and Bioinformatics, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia.; Babenko VA; A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia.; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, 117997 Moscow, Russia.; Tvorogova AV; Biological Faculty, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia.; Plotnikov EY; A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia.; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, 117997 Moscow, Russia.; Sukhikh GT; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, 117997 Moscow, Russia.; Zorov DB; A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia.; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, 117997 Moscow, Russia. |
| Source: | International journal of molecular sciences [Int J Mol Sci] 2026 Feb 10; Vol. 27 (4). Date of Electronic Publication: 2026 Feb 10. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Basel, Switzerland : MDPI, [2000- |
| MeSH Terms: | Nestin*/metabolism ; Nestin*/genetics ; Kidney*/metabolism ; Kidney*/cytology ; Kidney*/ultrastructure ; Stem Cells*/metabolism ; Stem Cells*/ultrastructure ; Stem Cells*/cytology; Green Fluorescent Proteins/metabolism ; Green Fluorescent Proteins/genetics ; Epithelial Cells/metabolism ; Epithelial Cells/ultrastructure ; Animals ; Mice ; Cells, Cultured |
| Abstract: | In this study, in a culture of renal epithelial cells, we identified those expressing nestin, a cytoskeletal protein associated with stem/progenitor/activated/proliferating cell states. A mouse expressing GFP under the nestin promoter was used, followed by cell isolation and culture. It is hypothesized that this can be used to assess the stem/progenitor/activated/proliferating cell level in a mixed kidney cell culture. Both nestin-positive and nestin-negative cells were demonstrated to be present in the culture. After visualization, cells were attached to a glass slide with a grid, fixed, and prepared for electron microscopy analysis, with each cell visually identified by light microscopy being analyzed. Electron microscopy revealed tight interactions between nestin-positive and nestin-negative cells. Significant differences in the ultrastructure of nestin-positive and nestin-negative cells were observed. Nestin-positive cells were distinguished by a high ribosome content, indicating high protein-synthesizing activity. In the nestin-GFP-high (sorted) population examined by electron microscopy, vesicle-containing protrusions were frequently observed. These cells could contain multiple nuclei of varying sizes and had a high content of lysosomes. No significant differences in mitochondrial ultrastructure were observed in nestin-positive and -negative cells, although functional characteristics evaluated by the membrane potential probe differed. |
| Grant Information: | 25-14-00114 Russian Science Foundation |
| Contributed Indexing: | Keywords: mitochondrial membrane potential; nestin; segmentation; stem cells; ultrastructure; vesicles |
| Substance Nomenclature: | 0 (Nestin); 147336-22-9 (Green Fluorescent Proteins); 0 (Nes protein, mouse) |
| Entry Date(s): | Date Created: 20260227 Date Completed: 20260306 Latest Revision: 20260306 |
| Update Code: | 20260306 |
| PubMed Central ID: | PMC12940724 |
| DOI: | 10.3390/ijms27041695 |
| PMID: | 41751832 |
| Database: | MEDLINE |
Journal Article