Portable and point-of-care molecular detection of pathogenic Vibrio parahaemolyticus in shrimp.
| Title: | Portable and point-of-care molecular detection of pathogenic Vibrio parahaemolyticus in shrimp. |
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| Authors: | Trinh TND; BioTechnology Institute, Tra Vinh University, Vinh Long Province, Vietnam.; Trinh KTL; Advanced Materials Technology Institute Vietnam National University Ho Chi Minh City, Ho Chi Minh City 70000, Viet Nam.; Viet Nam National University, Ho Chi Minh City 70000, Viet Nam.; Nguyen HA; Department of Molecular Biology, Institute of Food and Biotechnology, Can Tho University, Can Tho City, Viet Nam.; Nguyen HMT; Department of BioNano Technology, Gachon University, 1342 Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, 13120, South Korea. nylee@gachon.ac.kr.; Nhi NTH; School of Agriculture and Aquaculture, Tra Vinh University, Vinh Long Province, Vietnam.; Minh VTT; School of Agriculture and Aquaculture, Tra Vinh University, Vinh Long Province, Vietnam.; Nam NN; Biotechnology Center, School of Agriculture and Aquaculture, Tra Vinh University, Vinh Long Province, Vietnam.; Lee NY; Department of BioNano Technology, Gachon University, 1342 Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, 13120, South Korea. nylee@gachon.ac.kr. |
| Source: | The Analyst [Analyst] 2026 Apr 07. Date of Electronic Publication: 2026 Apr 07. |
| Publication Model: | Ahead of Print |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 0372652 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1364-5528 (Electronic) Linking ISSN: 00032654 NLM ISO Abbreviation: Analyst Subsets: MEDLINE |
| Imprint Name(s): | Publication: Cambridge : Royal Society of Chemistry; Original Publication: London : Chemical Society |
| Abstract: | In the Mekong Delta region, Acute Hepatopancreatic Necrosis Disease (AHPND) is a globally serious threat to shrimp farming. AHPND is mainly caused by Vibrio parahaemolyticus carrying a plasmid that encodes virulence genes, namely, Photorhabdus insect-related (pir). One of the best measures to control outbreaks in shrimp is to rapidly and accurately determine V. parahaemolyticus carrying virulence gene. In this study, we developed a fully-integrated molecular diagnostic device that combines a Flinders Technology Associates (FTA) card-embedded tube for nucleic acid extraction and loop-mediated isothermal amplification (LAMP) to detect the pirA gene of causative V. parahaemolyticus, with a pH-based colorimetric readout realized by the use of phenol red. To improve the usability of the LAMP assay, a palm-sized three-dimensional (3D)-printed heater operated by batteries was employed to apply heat for the amplification reaction on site. The strategy showed high specificity and sensitivity, with a limit of detection as low as 102 CFU mL-1. The assay can be completed within 75 min at 65 °C. Next, to prove the feasibility of the test for real samples, shrimp collected from a shrimp farm were used and spiked with bacteria. The strategy was proven to be capable of performing three main steps of molecular diagnosis such as sample extraction, amplification, and detection at the point of care for detecting the toxic pirA gene in V. parahaemolyticus. This strategy can also serve as a cost-effective and rapid tool and can be a potential candidate for on-site disease control in low-resource areas. |
| Entry Date(s): | Date Created: 20260407 Latest Revision: 20260514 |
| Update Code: | 20260514 |
| DOI: | 10.1039/d6an00099a |
| PMID: | 41945402 |
| Database: | MEDLINE |
Journal Article