Emergence of mobile RND-type efflux pump gene cluster tmexCD-toprJ on a broad-host-range IncC plasmid in Shewanella species in a water environment.
| Title: | Emergence of mobile RND-type efflux pump gene cluster tmexCD-toprJ on a broad-host-range IncC plasmid in Shewanella species in a water environment. |
|---|---|
| Authors: | Dao DT; Graduate School of Medicine, Nagoya University, Nagoya, Japan.; Takemura T; Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan.; Kasuga I; Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan; Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo, Japan; Graduate School of Engineering, The University of Tokyo, Japan.; Hirabayashi A; Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Japan Institute for Health Security, Tokyo, Japan.; Nga NT; Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan.; Anh PHQ; Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan.; Tu ND; National Institute of Hygiene and Epidemiology, Hanoi, Vietnam.; Trang LT; National Institute of Hygiene and Epidemiology, Hanoi, Vietnam.; Thai PD; National Institute of Hygiene and Epidemiology, Hanoi, Vietnam.; Tran HH; National Institute of Hygiene and Epidemiology, Hanoi, Vietnam.; Abe H; Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan.; Hasebe F; Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan.; Shibayama K; Graduate School of Medicine, Nagoya University, Nagoya, Japan.; Suzuki M; Graduate School of Medicine, Nagoya University, Nagoya, Japan; Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan; Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Japan Institute for Health Security, Tokyo, Japan. Electronic address: suzuki.mas@jihs.go.jp. |
| Source: | Journal of global antimicrobial resistance [J Glob Antimicrob Resist] 2026 Apr 17. Date of Electronic Publication: 2026 Apr 17. |
| Publication Model: | Ahead of Print |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Published by Elsevier Ltd. on behalf of International Society of Chemotherapy for Infection and Cancer Country of Publication: Netherlands NLM ID: 101622459 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2213-7173 (Electronic) Linking ISSN: 22137165 NLM ISO Abbreviation: J Glob Antimicrob Resist Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam : Published by Elsevier Ltd. on behalf of International Society of Chemotherapy for Infection and Cancer |
| Abstract: | Objectives: The spread of the mobile resistance-nodulation-division (RND)-type efflux pump tmexCD-toprJ gene cluster that confers multidrug resistance (MDR), including tigecycline resistance, in gram-negative bacteria poses a global public health threat. However, the occurrence of such clinically important antimicrobial resistance genes (ARGs) in the natural environments has not yet been well investigated. In this study, we investigated MDR aquatic bacteria in Vietnam.; Methods: A carbapenem- and tigecycline-resistant Shewanella xiamenensis isolate NUITM-VS2 was obtained from urban drainage in Hanoi, Vietnam, in October 2021. Antimicrobial susceptibility testing using agar dilution and whole-genome sequencing (WGS) was performed using both long-read and short-read sequencers. ARGs and plasmid replicons were detected using ResFinder and PlasmidFinder with the custom sequence database.; Results: NUITM-VS2 showed resistance to most antimicrobials tested, including tigecycline, tetracyclines, carbapenems, cephalosporins, fluoroquinolone, and aminoglycosides. WGS analysis resulted in the complete genome sequence consisting of one chromosome and five plasmids. A 152.2-kb IncC plasmid, pNUITM-VS2_2, co-carried two mobile tigecycline resistance genes, tet(X4) and tmexCD3-toprJ1b-like gene cluster. In addition, a 24.8-kb untypeable plasmid, pNUITM-VS2_4, carried the carbapenemase gene blaNDM-1. pNUITM-VS2_2 was transferred to Escherichia coli by conjugation, which simultaneously conferred high-level resistance against many antimicrobials, including tigecycline.; Conclusion: To the best of our knowledge, this is the first report of the detection of the mobile RND-type efflux pump gene cluster tmexCD-toprJ in Shewanella species. Our results provide genetic evidence of the complexity of the dynamics of clinically important ARGs among aquatic bacteria, which could be important reservoirs for ARGs in the natural environment.; (Copyright © 2026 The Author(s). Published by Elsevier Ltd.. All rights reserved.) |
| Competing Interests: | Declaration of competing interest None declared. |
| Contributed Indexing: | Keywords: Shewanella; bla(NDM-1); carbapenem; tigecycline; tmexCD-toprJ |
| Entry Date(s): | Date Created: 20260419 Latest Revision: 20260419 |
| Update Code: | 20260420 |
| DOI: | 10.1016/j.jgar.2026.04.005 |
| PMID: | 42002144 |
| Database: | MEDLINE |
Journal Article