| Title: |
A Neutralizing Antibody Selected from Plasma Cells That Binds to Group 1 and Group 2 Influenza A Hemagglutinins. |
| Authors: |
Corti, Davide; Voss, arrod; Gamblin, Steven J.; Codoni, Giosiana; Macagno, Annalisa; arrossay, David; Vachieri, Sebastien G.; Pinna, Debora; Minola, Andrea; Vanzetta, Fabrizia; Silacci, Chiara; Fernandez-Rodriguez, Blanca M.; Agatic, Gloria; Bianchi, Siro; Giacchetto-Sasselli, Isabella; Calder, Lesley; Sallusto, Federica; Collins, Patrick; Haire, Lesley F.; Temperton, Nigel |
| Source: |
Science (pre-March 2025); 8/12/2011, Vol. 333 Issue 6044, p850-856, 7p |
| Subject Terms: |
Immunology; Influenza A virus; Immunoglobulins; Research methodology; Cell culture; Monoclonal antibodies; Hemagglutinin; X-ray crystallography; Epitopes; Vaccines |
| Abstract: |
The isolation of broadly neutralizing antibodies against influenza A viruses has been a long-sought goal for therapeutic approaches and vaccine design. Using a single-cell culture method for screening large numbers of human plasma cells, we isolated a neutralizing monoclonal antibody that recognized the hemagglutinin (HA) glycoprotein of all 16 subtypes and neutralized both group 1 and group 2 influenza A viruses. Passive transfer of this antibody conferred protection to mice and ferrets. Complexes with HAs from the group 1 H1 and the group 2 H3 subtypes analyzed by x-ray crystallography showed that the antibody bound to a conserved epitope in the F subdomain. This antibody may be used for passive protection and to inform vaccine design because of its broad specificity and neutralization potency. [ABSTRACT FROM AUTHOR] |
| : |
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| Database: |
Complementary Index |