| Title: |
Molecular Structure from Models to Mastery: An Inquiry-Based Study of Human Insulin-Degrading Enzyme |
| Language: |
English |
| Authors: |
Michael A. Araujo; Alexandra A. Barrere; Selena-Rae Tirado; Candace E. Williams; Monica I. Strada; Benjamin J. Alper (ORCID 0000-0002-7940-9655) |
| Source: |
Journal of Chemical Education. 2022 99(12):4094-4101. |
| Availability: |
Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc |
| Peer Reviewed: |
Y |
| Page Count: |
8 |
| Publication Date: |
2022 |
| Document Type: |
Journal Articles; Reports - Research |
| Education Level: |
Higher Education; Postsecondary Education |
| Descriptors: |
Molecular Structure; Science Instruction; Science Laboratories; Science Experiments; Laboratory Experiments; Undergraduate Students; College Science; Biochemistry |
| DOI: |
10.1021/acs.jchemed.1c01285 |
| ISSN: |
0021-9584; 1938-1328 |
| Abstract: |
Using crystal structure data, site directed mutagenesis, and real-time kinetic assays, students designed, expressed, and purified engineered mutants of human insulin-degrading enzyme (IDE). Students designed their own IDE mutants following "in silico" analysis, used inverse PCR to generate mutant plasmid expression constructs, expressed mutant proteins in "E. coli", and tested their function "in vitro." Structural requirements for enzyme function were investigated. Students demonstrated a mastery of critical concepts in enzymology including principles of experimental design and evolutionary implications of enzyme structure-function relationships. Experimental methods for the analysis of macromolecular interactions and computational approaches to scientific inquiry were explored and applied. This investigation was conducted as part of the second-semester undergraduate biochemistry laboratory at Sacred Heart University. |
| Abstractor: |
As Provided |
| Entry Date: |
2024 |
| Accession Number: |
EJ1441710 |
| Database: |
ERIC |