| Title: |
P-785. Pooled Antibiotic Susceptibility Testing (P-AST) Reveals High Prevalence of Multidrug- Resistance in Pediatric UTI Which Was Underreported by Standard Isolate Susceptibility Testing |
| Authors: |
Bhavsar, Sejal M; Polavarapu, Nisha; Haley, Emery D; Luke, Natalie; Mathur, Mohit; Chen, Xiaofei; Havrilla, Jim; Baunoch, David A; Lieberman, Kenneth V |
| Source: |
Open Forum Infectious Diseases ; volume 13, issue Supplement_1 ; ISSN 2328-8957 |
| Publisher Information: |
Oxford University Press (OUP) |
| Publication Year: |
2026 |
| Description: |
Background Pediatric urinary tract infections (UTIs) can result in both acute complications, such as pyelonephritis or urosepsis, and chronic health complications, including recurrent UTI and renal damage. Therefore, rapid diagnosis and appropriate treatment of pediatric UTIs are essential.Figure 1.Frequency of Resistance Genes DetectedTable 1.Side-by-Side Comparison of Antibiotic Susceptibility Results Between Standard Isolate Susceptibility Testing and P-AST MethodsSUC = Standard Urine Culture; AST = Antibiotic Susceptibility Testing; M-PCR = Multiplex-Polymerase Chain Reaction; P-AST = Pooled Antibiotic Susceptibility Testing. Available SUC/Isolate AST results only included a list of antibiotics with “susceptible” results; P-AST reports included both lists of antibiotics with “susceptible” results and “resistant” results. Antibiotics listed in red were reported as susceptible by SUC/Isolate AST but as resistant by P-AST; Penicillin and Aztreonam (blue) were reported via SUC/Isolate AST but were not included in the P-AST assay. Methods A previous study demonstrated superiority of Multiplex Polymerase Chain Reaction (M-PCR) over standard urine culture (SUC) in detecting microorganisms in the urine of 44 female and four male patients aged 3– 21 years old presenting to a Pediatric Emergency Department with clinically suspected UTI. Using the same cohort, this analysis explores the occurrence of phenotypic and/or molecular resistance detection by Pooled Antibiotic Susceptibility Testing (P-AST) and M-PCR in specimens with susceptible results from isolate testing. Results M-PCR was positive for UTI pathogens in 42 specimens and P-AST was performed on 36 of these. Of the 36 tested by P-AST, resistance genes were also detected in 18 (Figure 1). The concordance between resistance gene detection and phenotypic resistance by P-AST was ≈ 60%. Two specimens that were positive for S. aureus had methicillin resistance genes detected, but both were negative for the colorimetric MRSA phenotype assay. No vancomycin or ... |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1093/ofid/ofaf695.996 |
| Availability: |
https://doi.org/10.1093/ofid/ofaf695.996; https://academic.oup.com/ofid/article-pdf/13/Supplement_1/ofaf695.996/66349872/ofaf695.996.pdf |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.110E066A |
| Database: |
BASE |