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A chromosome-level reference genome for the critically endangered Southern Corroboree frog (Pseudophryne corroboree)

Title: A chromosome-level reference genome for the critically endangered Southern Corroboree frog (Pseudophryne corroboree)
Authors: Kosch, TA; Berger, L; Crawford, AJ; Gilbert, D; Goodall, D; Hunter, D; McFadden, M; Abueg, L; Formenti, G; Collins, J; Tilley, T; Jain, N; O'Toole, B; Whelan, C; Cooke, I; Muffato, M; Yates, B; Fedrigo, O; Balacco, J; Jarvis, ED; Skerratt, LF
Publisher Information: Wellcome
Publication Year: 2025
Collection: The University of Melbourne: Digital Repository
Description: The Southern Corroboree frog (Pseudophryne corroboree; Anura; Myobatrachidae) is a Critically Endangered amphibian, according to the IUCN, and is endemic to the Snowy Mountains region of Kosciuszko National Park in New South Wales, Australia. This species has been driven to functional extinction by the introduction of the fungal disease, chytridiomycosis. Here we provide the first reference genome for P. corroboree. Using PacBio HiFi sequencing, Arima Hi-C, and Bionano optical mapping, we produced a chromosome-level genome assembly. Additionally, we generated a reference transcriptome based on multiple tissues from both male and female individuals to support genome annotation. The resulting genome spans 8.87 Gb across 12 chromosomes, with a contig N50 of 6.8 Mb. This research provides a phased, annotated genome assembly along with transcriptomic resources to facilitate future conservation genomic studies of P. corroboree. Furthermore, the genome offers an invaluable resource for taxonomic and evolutionary research, particularly given the nearest available chromosome-level reference genome is from Mixophyes fleayi, a species that last shared a common ancestor with P. corroboree 80 million years ago.
Document Type: article in journal/newspaper
Language: English
ISSN: 2398-502X
Relation: https://hdl.handle.net/11343/365255
Availability: https://hdl.handle.net/11343/365255
Rights: https://creativecommons.org/licenses/by/4.0 ; CC BY
Accession Number: edsbas.133B05DA
Database: BASE