| Title: |
815 Inhalation Injury Is Associated with a Unique Cytokine Profile in Burn Patients |
| Authors: |
Vitharana, Dhanushka; Trinh, Sophia; Deville, Paige; Masoud, Abdul-Razak; Ozcan, Ada; Andre, Kaitlyn; Carter, Jeffrey; Phelan, Herbert; Schoen, Jonathan; Miles, Victoria; Smith, Alison |
| Source: |
Journal of Burn Care & Research ; volume 46, issue Supplement_1, page S267-S267 ; ISSN 1559-047X 1559-0488 |
| Publisher Information: |
Oxford University Press (OUP) |
| Publication Year: |
2025 |
| Description: |
Introduction Inhalation injuries from burns are associated with worse patient outcomes including increased risk of pneumonia and progression to multisystem organ failure. Increased concentrations of several pro- and anti-inflammatory cytokines have been found in non-survivors of inhalation injuries compared to survivors. The aim of this study was to examine how the inflammatory profile of burn patients with inhalation injuries differs from those without inhalation injuries. It is hypothesized that patients with inhalation injuries will demonstrate a different cytokine profile to burn patients without inhalation injuries. Methods After obtaining IRB approval, adipose tissue was collected from adult burn patients presenting to an ABA-Verified Burn Center from 2022-2024 during initial excision. Thermal, electrical and chemical burns were included, as well as burn patients with concomitant trauma. Adipose-derived stem cells (ADSCs) were extracted and the supernatant was collected. Cytokine analysis was performed using a 10-analyte multiplex assay. The cytokines studied were IFN-γ, IL-1β, IL-4, IL-6, IL-10, IL-13, IL-17A, TGF-α, TNF-α, FGF-2, MCP-1 and VEGF. Patients were stratified based on the presence of inhalation injury. Statistical analysis was performed using the Mann-Whitney U test. Results Of the 28 patients studied, 15 patients (54%) sustained inhalation injuries. No significant differences in age, body mass index, sex, % total body surface area burn, blood alcohol level on admission, smoking status or mortality were observed between groups (p>0.05). In patients with inhalation injuries, IL-8 was significantly decreased as compared to patients without inhalation injury (p=0.03). No significant differences in the other cytokines analyzed were identified between groups (p>0.05). Conclusions This study suggests that adipose derived stem cells from burn patients with inhalation injury produce decreased levels of IL-8 in adipose tissue. IL-8 is a pro-inflammatory cytokine that activates ... |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1093/jbcr/iraf019.346 |
| Availability: |
https://doi.org/10.1093/jbcr/iraf019.346; https://academic.oup.com/jbcr/article-pdf/46/Supplement_1/S267/62823027/iraf019.346.pdf |
| Rights: |
http://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.1344579F |
| Database: |
BASE |