| Title: |
Proteomic profiling of the local and systemic immune response to pediatric respiratory viral infections |
| Authors: |
Lydon, Emily; Osborne, Christina M; Wagner, Brandie D; Ambroggio, Lilliam; Harris, J Kirk; Reeder, Ron; Carpenter, Todd C; Maddux, Aline B; Leroue, Matthew K; Yehya, Nadir; DeRisi, Joseph L; Hall, Mark W; Zuppa, Athena F; Carcillo, Joseph; Meert, Kathleen; Sapru, Anil; Pollack, Murray M; McQuillen, Patrick; Notterman, Daniel A; Langelier, Charles R; Mourani, Peter M; Network, for the Eunice Kennedy Shriver National Institute of Child Health and Human Development Collaborative Pediatric Critical Care Research |
| Contributors: |
Garg, Neha |
| Source: |
mSystems, vol 10, iss 1 |
| Publisher Information: |
eScholarship, University of California |
| Publication Year: |
2025 |
| Collection: |
University of California: eScholarship |
| Subject Terms: |
3207 Medical Microbiology (for-2020); 32 Biomedical and Clinical Sciences (for-2020); Lung (rcdc); Clinical Research (rcdc); Infectious Diseases (rcdc); Biotechnology (rcdc); Pediatric (rcdc); 2.1 Biological and endogenous factors (hrcs-rac); Infection (hrcs-hc); 3 Good Health and Well Being (sdg); Humans (mesh); Male (mesh); Proteomics (mesh); Female (mesh); Respiratory Tract Infections (mesh); Child; Preschool (mesh); Child (mesh); Infant (mesh); Proteome (mesh); Virus Diseases (mesh); Viral Load (mesh); Biomarkers (mesh); proteomics; LRTI; pneumonia; host response; co-infection; viral pneumonia; respiratory viruses |
| Subject Geographic: |
e01335 - e01324 |
| Description: |
Viral lower respiratory tract infection (vLRTI) is a leading cause of hospitalization and death in children worldwide. Despite this, no studies have employed proteomics to characterize host immune responses to severe pediatric vLRTI in both the lower airway and systemic circulation. To address this gap, gain insights into vLRTI pathophysiology, and test a novel diagnostic approach, we assayed 1,305 proteins in tracheal aspirate (TA) and plasma from 62 critically ill children using SomaScan. We performed differential expression (DE) and pathway analyses comparing vLRTI (n = 40) to controls with non-infectious acute respiratory failure (n = 22), developed a diagnostic classifier using LASSO regression, and analyzed matched TA and plasma samples. We further investigated the impact of viral load and bacterial coinfection on the proteome. The TA signature of vLRTI was characterized by 200 DE proteins (Padj |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
unknown |
| Relation: |
qt9kq2267g; https://escholarship.org/uc/item/9kq2267g; https://escholarship.org/content/qt9kq2267g/qt9kq2267g.pdf |
| DOI: |
10.1128/msystems.01335-24 |
| Availability: |
https://escholarship.org/uc/item/9kq2267g; https://escholarship.org/content/qt9kq2267g/qt9kq2267g.pdf; https://doi.org/10.1128/msystems.01335-24 |
| Rights: |
CC-BY |
| Accession Number: |
edsbas.134FFEEF |
| Database: |
BASE |