| Title: |
Exogenous paraoxonase‐1 during oocyte maturation improves bovine embryo development in vitro |
| Authors: |
Rincón, JAA; Madeira, EM; Campos, FT; Mion, B; Silva, JF; Absalón‐Medina, VA; Butler, WR; Corrêa, MN; Pegoraro, LMC; Schneider, A |
| Contributors: |
Conselho Nacional de Desenvolvimento Científico e Tecnológico; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul |
| Source: |
Reproduction in Domestic Animals ; volume 51, issue 5, page 827-830 ; ISSN 0936-6768 1439-0531 |
| Publisher Information: |
Wiley |
| Publication Year: |
2016 |
| Collection: |
Wiley Online Library (Open Access Articles via Crossref) |
| Description: |
Contents Paraoxonase‐1 ( PON 1) is an enzyme found in serum and follicular fluid that protects cell membrane and circulating lipids against oxidative damage. The aims of this study were to measure the direct effects of recombinant PON 1 ( rPON 1) on bovine oocyte maturation at the molecular level (gene expression) and to measure the carry‐over effects of PON 1 on pre‐implantation embryo development in vitro. COC s were submitted to IVM with the addition of 0.0, 0.02, 0.04 and 0.08 mg ml −1 of rPON 1, corresponding to an average PON 1 arylesterase enzyme activity of 2.2 ± 0.4, 15.5 ± 1.5, 30.2 ± 3.0 and 57.9 ± 5.0 U ml −1 , respectively. The results indicated that addition of rPON 1 during IVM improved embryo development in a dose‐dependent manner as D7 embryo development was 22.2%, 29.4%, 32.2% and 37.0% for the treatment groups, respectively ( p = 0.02). In conclusion, addition of PON 1 enzyme during IVM exerted dose‐related positive effects on embryo development rates to blastocysts. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1111/rda.12730 |
| Availability: |
https://doi.org/10.1111/rda.12730; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Frda.12730; https://onlinelibrary.wiley.com/doi/pdf/10.1111/rda.12730 |
| Rights: |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
| Accession Number: |
edsbas.13F03FFE |
| Database: |
BASE |