| Title: |
The Long-Term Prognostic Significance of Circulating Tumor Cells in Ovarian Cancer-A Study of the OVCAD Consortium |
| Authors: |
Obermayr, Eva; Reiner, Angelika; Brandt, Burkhard; Braicu, Elena Ioana; Reinthaller, Alexander; Loverix, Liselore; Concin, Nicole; Woelber, Linn; Mahner, Sven; Sehouli, Jalid; Vergote, Ignace; Zeillinger, Robert |
| Source: |
ISSN:2072-6694 ; Cancers, vol. 13 (11), Art.No. ARTN 2613. |
| Publisher Information: |
Multidisciplinary Digital Publishing Institute (MDPI) |
| Publication Year: |
2021 |
| Subject Terms: |
Science & Technology; Life Sciences & Biomedicine; Oncology; primary epithelial ovarian cancer; circulating tumor cells; long-term survival; MOLECULAR CHARACTERIZATION; BREAST-CANCER; EXPRESSION; METASTASIS; SURVIVORS; MARKERS; MODELS; 1112 Oncology and Carcinogenesis; 3211 Oncology and carcinogenesis |
| Description: |
INTRODUCTION: We previously reported the prognostic impact of circulating tumor cells (CTCs) in a multicenter study on minimal residual disease in primary ovarian cancer. With additional follow-up data, we evaluated the combined CTC approach (CTCscombo), in particular for the patients who had survived more than five years. MATERIAL AND METHODS: Blood samples taken at baseline and six months after adjuvant treatment (follow-up) were assessed by quantitative PCR (qPCR) measuring PPIC transcripts and immunofluorescent staining (IF). A positive result with either IF or qPCR was classified as CTCcombo-positive. Further, PPIC was assessed in the primary tumor tissue. RESULTS: The concordance of IF and qPCR was 65% at baseline and 83% after treatment. Results showed that 50.5% of the baseline and 29.5% of the follow-up samples were CTCcombo-positive. CTCscombo after treatment were associated with increased mortality after adjusting for FIGO stage (HR 2.574, 95% CI: 1.227-5.398, p = 0.012), a higher risk of recurrence after adjusting for peritoneal carcinosis (HR 4.068, 95% CI: 1.948-8.498, p < 0.001), and increased mortality after five survived years. DISCUSSION: The two-sided analytical approach revealed CTC subpopulations associated with ovarian cancer progression and may illuminate a potential treatment-related shift in molecular phenotypes. That approach can identify patients who have elevated risk of recurrence and death due to ovarian cancer and who may require risk-adapted treatment strategies. ; sponsorship: This research was funded by the Sixth Framework Programme FP6-LIFESCIHEALTH (Project "OVCAD-Diagnosis of a Silent Killer", project no LSHC-CT-2005-018698). (Sixth Framework Programme FP6-LIFESCIHEALTH (Project "OVCAD-Diagnosis of a Silent Killer")|LSHC-CT-2005-018698) ; status: Published |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
https://lirias.kuleuven.be/handle/123456789/680897; https://doi.org/10.3390/cancers13112613; https://pubmed.ncbi.nlm.nih.gov/34073412 |
| DOI: |
10.3390/cancers13112613 |
| Availability: |
https://lirias.kuleuven.be/handle/123456789/680897; https://lirias.kuleuven.be/retrieve/1d8b6efa-a9bf-4b56-9c8c-64b615f30d16; https://doi.org/10.3390/cancers13112613; https://pubmed.ncbi.nlm.nih.gov/34073412 |
| Rights: |
info:eu-repo/semantics/openAccess ; public ; All rights reserved |
| Accession Number: |
edsbas.142E90FF |
| Database: |
BASE |