| Title: |
Near-care assay of plasma glial fibrillary acid protein and ubiquitin carboxyl-terminal hydrolase isozyme L1 with shorter and prolonged duration exercise |
| Authors: |
Stacey, M; Barden, A; Snape, D; Wainwright, B; Parsons, I; Leckie, T; Fitzpatrick, D; Pitsiladis, Y; Palin, T; O'Hara, J; Woods, D |
| Publisher Information: |
Nature Portfolio |
| Publication Year: |
2026 |
| Collection: |
Leeds Beckett University Repository |
| Description: |
Background and Objectives: Neurobiomarkers measured in peripheral blood can supplement management strategies following traumatic brain injury (TBI). Dual-assay of glial fibrillary acid protein (GFAP) and ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) is FDA-approved to inform a decision threshold approach (GFAP >30 μg.L-1 and/or UCHL1 >360 μg.L-1) for post-TBI neuroimaging. As physical activity and thermal strain often accompany TBI-prone activities, we investigated whether each molecule’s quantification - and, by extension, clinical decisions - could be influenced by exercise-heat stress. Methods: In healthy volunteers monitored continuously for body core temperature (Tc), we used the i-STAT Alinity to assess plasma GFAP and UCHL1 responses to exercise in the laboratory (four female, eighteen male trained participants, cycling for 45 min in 32 °C) and field (three female and 22 male recreational marathon runners, finishing time 231± 34 min, peak ambient temperature 11 °C). Results: Respective ΔTc overall were 1.42 ± 0.37 °C and 1.87 [1.53, 2.31] °C. With laboratory exercise, GFAP and UCHL1 did not exceed the manufacturer’s decision threshold. Across the marathon, GFAP was stable, whereas UCH-L1 more than doubled (200 [200, 200] vs 462 [310, 782] μg.L-1, P |
| Document Type: |
article in journal/newspaper |
| File Description: |
text |
| Language: |
English |
| ISSN: |
2045-2322 |
| Relation: |
https://eprints.leedsbeckett.ac.uk/id/eprint/12740/1/NearcareAssayOfPlasmaGlialFibrillaryAcidProteinAndUbiquitinCarboxylterminalHydrolaseIsozymeL1WithShorterAndProlongedDurationExerciseAM-O%27HARA.pdf; Stacey, M and Barden, A and Snape, D and Wainwright, B and Parsons, I and Leckie, T and Fitzpatrick, D and Pitsiladis, Y and Palin, T and O'Hara, J and Woods, D (2026) Near-care assay of plasma glial fibrillary acid protein and ubiquitin carboxyl-terminal hydrolase isozyme L1 with shorter and prolonged duration exercise. Scientific Reports. ISSN 2045-2322 (In Press) |
| Availability: |
https://eprints.leedsbeckett.ac.uk/id/eprint/12740/ |
| Accession Number: |
edsbas.150B22F0 |
| Database: |
BASE |