| Title: |
Genetic effects on the skin methylome in healthy older twins. |
| Authors: |
Shore, CJ; Villicaña, S; El-Sayed Moustafa, JS; Roberts, AL; Gunn, DA; Bataille, V; Deloukas, P; Spector, TD; Small, KS; Bell, JT |
| Publication Year: |
2024 |
| Collection: |
Queen Mary University of London: Queen Mary Research Online (QMRO) |
| Subject Terms: |
DNA methylation; QTLs; gene expression; heritability; skin; Humans; Female; Quantitative Trait Loci; CpG Islands; Epigenome; Aged; Genome-Wide Association Study; Middle Aged; Twins; Monozygotic; Melanoma; United Kingdom; Epigenesis; Genetic |
| Description: |
Whole-skin DNA methylation variation has been implicated in several diseases, including melanoma, but its genetic basis has not yet been fully characterized. Using bulk skin tissue samples from 414 healthy female UK twins, we performed twin-based heritability and methylation quantitative trait loci (meQTL) analyses for >400,000 DNA methylation sites. We find that the human skin DNA methylome is on average less heritable than previously estimated in blood and other tissues (mean heritability: 10.02%). meQTL analysis identified local genetic effects influencing DNA methylation at 18.8% (76,442) of tested CpG sites, as well as 1,775 CpG sites associated with at least one distal genetic variant. As a functional follow-up, we performed skin expression QTL (eQTL) analyses in a partially overlapping sample of 604 female twins. Colocalization analysis identified over 3,500 shared genetic effects affecting thousands of CpG sites (10,067) and genes (4,475). Mediation analysis of putative colocalized gene-CpG pairs identified 114 genes with evidence for eQTL effects being mediated by DNA methylation in skin, including in genes implicating skin disease such as ALOX12 and CSPG4. We further explored the relevance of skin meQTLs to skin disease and found that skin meQTLs and CpGs under genetic influence were enriched for multiple skin-related genome-wide and epigenome-wide association signals, including for melanoma and psoriasis. Our findings give insights into the regulatory landscape of epigenomic variation in skin. |
| Document Type: |
article in journal/newspaper |
| File Description: |
1932 - 1952 |
| Language: |
English |
| Relation: |
Am J Hum Genet; https://qmro.qmul.ac.uk/xmlui/handle/123456789/100548 |
| DOI: |
10.1016/j.ajhg.2024.07.010 |
| Availability: |
https://qmro.qmul.ac.uk/xmlui/handle/123456789/100548; https://doi.org/10.1016/j.ajhg.2024.07.010 |
| Rights: |
This is an open access article distributed under the terms of the Creative Commons CC-BY license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. ; © 2024 The Authors. |
| Accession Number: |
edsbas.1527E6A9 |
| Database: |
BASE |