| Title: |
Trypanosoma brucei gambiense group 2 experimental in vivo life cycle: from procyclic to bloodstream form ; Cycle de vie expérimental in vivo de Trypanosoma brucei gambiense groupe 2 : de la forme procyclique à la forme sanguicole |
| Authors: |
Juban, Paola; Bart, Jean-Mathieu; Ségard, Adeline; Jamonneau, Vincent; Ravel, Sophie |
| Contributors: |
Interactions hôtes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatides (UMR INTERTRYP); Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université Claude Bernard Lyon 1 (UCBL); Université de Lyon-Université de Lyon-Université de Bordeaux (UB); This work was supported by the IRD (Institut de Recherche pour le Développement). Paola Juban was supported by the KIM RIVE project (Key Initiative Muse Risks & Vectors) from Montpellier Université d’excellence (MUSE). |
| Source: |
ISSN: 1252-607X. |
| Publisher Information: |
CCSD; EDP Sciences |
| Publication Year: |
2024 |
| Collection: |
HAL Lyon 1 (University Claude Bernard Lyon 1) |
| Subject Terms: |
Trypanosoma brucei gambiense; Procyclic form; Bloodstream form; Glossina; Life cycle; [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology |
| Description: |
International audience ; Trypanosoma brucei gambiense (Tbg) group 2 is a subgroup of trypanosomes able to infect humans and is found in West and Central Africa. Unlike other agents causing sleeping sickness, such as Tbg group 1 and Trypanosoma brucei rhodesiense, Tbg2 lacks the typical molecular markers associated with resistance to human serum. Only 36 strains of Tbg2 have been documented, and therefore, very limited research has been conducted despite their zoonotic nature. Some of these strains are only available in their procyclic form, which hinders human serum resistance assays and mechanistic studies. Furthermore, the understanding of Tbg2’s potential to infect tsetse flies and mammalian hosts is limited. In this study, 165 Glossina palpalis gambiensis flies were experimentally infected with procyclic Tbg2 parasites. It was found that 35 days post-infection, 43 flies out of the 80 still alive were found to be Tbg2 PCR-positive in the saliva. These flies were able to infect 3 out of the 4 mice used for blood-feeding. Dissection revealed that only six flies in fact carried mature infections in their midguts and salivary glands. Importantly, a single fly with a mature infection was sufficient to infect a mammalian host. This Tbg2 transmission success confirms that Tbg2 strains can establish in tsetse flies and infect mammalian hosts. This study describes an effective in vivo protocol for transforming Tbg2 from procyclic to bloodstream form, reproducing the complete Tbg2 cycle from G. p. gambiensis to mice. These findings provide valuable insights into Tbg2’s host infectivity, and will facilitate further research on mechanisms of human serum resistance. ; Trypanosoma brucei gambiense (Tbg) groupe 2 est un sous-groupe de trypanosomes capables d’infecter l’Homme, présent en Afrique de l’Ouest et en Afrique centrale. Contrairement aux autres agents responsables de la maladie du sommeil, tels que Tbg groupe 1 et Trypanosoma brucei rhodesiense, Tbg2 ne présente pas les marqueurs moléculaires habituellement ... |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
IRD: fdi:010089750 |
| DOI: |
10.1051/parasite/2024009 |
| Availability: |
https://hal.science/hal-04518058; https://hal.science/hal-04518058v1/document; https://hal.science/hal-04518058v1/file/parasite230155.pdf; https://doi.org/10.1051/parasite/2024009 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.152FEFA4 |
| Database: |
BASE |