| Title: |
Cbx3 maintains lineage specificity during neural differentiation |
| Authors: |
Huang, Chengyang; Su, Trent; Xue, Yong; Cheng, Chen; Lay, Fides D; McKee, Robin A; Li, Meiyang; Vashisht, Ajay; Wohlschlegel, James; Novitch, Bennett G; Plath, Kathrin; Kurdistani, Siavash K; Carey, Michael |
| Source: |
Genes & Development, vol 31, iss 3 |
| Publisher Information: |
eScholarship, University of California |
| Publication Year: |
2017 |
| Collection: |
University of California: eScholarship |
| Subject Terms: |
Biological Sciences; Genetics; Neurosciences; Regenerative Medicine; Stem Cell Research - Embryonic - Non-Human; Stem Cell Research - Nonembryonic - Non-Human; Stem Cell Research; 1.1 Normal biological development and functioning; Animals; Cell Differentiation; Cell Lineage; Cells; Cultured; Chromosomal Proteins; Non-Histone; Cyclin-Dependent Kinase 8; Embryonic Stem Cells; Gene Expression Regulation; Mediator Complex; Mesoderm; Mice; Neural Stem Cells; Promoter Regions; Genetic; RNA; Small Interfering; Cbx3; Med26; embryonic stem cell; neural precursor |
| Subject Geographic: |
241 - 246 |
| Description: |
Chromobox homolog 3 (Cbx3/heterochromatin protein 1γ [HP1γ]) stimulates cell differentiation, but its mechanism is unknown. We found that Cbx3 binds to gene promoters upon differentiation of murine embryonic stem cells (ESCs) to neural progenitor cells (NPCs) and recruits the Mediator subunit Med26. RNAi knockdown of either Cbx3 or Med26 inhibits neural differentiation while up-regulating genes involved in mesodermal lineage decisions. Thus, Cbx3 and Med26 together ensure the fidelity of lineage specification by enhancing the expression of neural genes and down-regulating genes specific to alternative fates. |
| Document Type: |
article in journal/newspaper |
| Language: |
unknown |
| Relation: |
qt5b39v19j; https://escholarship.org/uc/item/5b39v19j |
| DOI: |
10.1101/gad.292169.116 |
| Availability: |
https://escholarship.org/uc/item/5b39v19j; https://doi.org/10.1101/gad.292169.116 |
| Rights: |
public |
| Accession Number: |
edsbas.1533BCD9 |
| Database: |
BASE |