| Title: |
Comparative genomic surveillance of carbapenem-resistant Acinetobacter baumannii in the Netherlands in 2015–2017 and 2022–2024 |
| Authors: |
Allewijn, Max P.; Witteveen, Sandra; Vendrik, Karuna E. W.; de Haan, Angela; Landman, Fabian; Bos, Jeroen; Lansu, Simon; Wielders, Cornelia C. H.; Schoffelen, Annelot F.; de Greeff, Sabine C.; Notermans, Daan W.; Hendrickx, Antoni P. A. |
| Contributors: |
Carabetta, Valerie Jean |
| Source: |
Microbiology Spectrum ; ISSN 2165-0497 |
| Publisher Information: |
American Society for Microbiology |
| Publication Year: |
2026 |
| Description: |
We investigated the genomic epidemiology of carbapenem-resistant Acinetobacter baumannii (CRAb) during two surveillance periods: 2015–2017 (pilot 1) and 2022–2024 (pilot 2) in an international context to identify potential circulating high-risk lineages in the Netherlands. A total of 204 CRAb isolates were all analyzed by the carbapenem inactivation method, meropenem Etest, and whole-genome sequencing (combining Illumina and Nanopore). Comparative resistome, multilocus sequence typing (MLST), and whole-genome MLST (wgMLST) analyses were performed between pilots and compared to 577 international CRAb genomes. The Dutch CRAb population remained genetically diverse and stable between pilots and was dominated by international clone (IC)2 (61.8%), followed by IC9 (11.3%), IC1 (6.4%), and IC6 (5.9%). Also, epidemiological and clinical characteristics remained stable across both pilots. In contrast, CRAb in patients from Ukraine was genetically diverse and an epidemiologically distinct group. wgMLST indicated an increase in genetic clustering in 2022–2024, with unique CRAb clusters associated with Ukrainian patients. Of 26 identified clusters, 69% were linked to international sources. Differences in resistomes were observed between CRAb in patients from the Netherlands and Ukraine, and between pilots. Overall, 75% of isolates harbored bla OXA-23 -like genes, and bla NDM genes increased from 16% to 21%. None of the CRAb isolates from Ukrainian patients carried bla NDM genes; instead, they harbored bla OXA-23 -like and bla OXA-24 -like genes. Hybrid assemblies showed that carbapenemase genes were predominantly chromosomal. In conclusion, CRAb in the Netherlands showed genetic stability over time, yet the presence of New Delhi metallo-β-lactamase-carbapenemases and import of strains from high-risk regions underline the necessity for ongoing national surveillance. IMPORTANCE In 2015–2017 and 2022–2024, pilot surveillance of carbapenem-resistant Acinetobacter baumannii (CRAb) was performed in the Netherlands. Three ... |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1128/spectrum.02602-25 |
| Availability: |
https://doi.org/10.1128/spectrum.02602-25; https://journals.asm.org/doi/pdf/10.1128/spectrum.02602-25 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ ; https://journals.asm.org/non-commercial-tdm-license |
| Accession Number: |
edsbas.156FA3C5 |
| Database: |
BASE |