| Title: |
Unveiling the Phenotypic Variability of Macrophages: Insights from Donor Diversity and Pooling Strategies |
| Authors: |
Bartłomiej Taciak; Agnieszka Grochowska; Małgorzata Górczak; Emilia Górka; Marcin Skorzynski; Maciej Białasek; Tomasz P. Rygiel; Magdalena Król |
| Source: |
International Journal of Molecular Sciences ; Volume 26 ; Issue 3 ; Pages: 1272 |
| Publisher Information: |
Multidisciplinary Digital Publishing Institute |
| Publication Year: |
2025 |
| Collection: |
MDPI Open Access Publishing |
| Subject Terms: |
macrophages; BMDM; donor diversity |
| Subject Geographic: |
agris |
| Description: |
Macrophages are key players in inflammation and immune responses due to their phenotypic plasticity. This study examined the effects of pooling donor-derived macrophages on their phenotype and function, focusing on murine bone marrow-derived macrophages (BMDMs) and human monocyte-derived macrophages (hMDMs). Murine BMDMs were generated using L929-conditioned media and compared across single and pooled donors (two-to-five mice). Similarly, hMDMs cultured with M-CSF from individual donors were compared to pooled cultures. Pooling macrophages did not alter core phenotypic markers (CD11b, F4/80, CD64) or functional outputs such as cytokine secretion and nitric oxide production. In hMDMs, pooling reduced variability and led to slightly elevated or more-uniform marker expression. These findings demonstrate that pooling macrophages minimizes inter-individual variability without compromising cellular stability or function, enhancing reproducibility in immunological research while maintaining the option of single-donor studies for personalized analyses. |
| Document Type: |
text |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
Molecular Immunology; https://dx.doi.org/10.3390/ijms26031272 |
| DOI: |
10.3390/ijms26031272 |
| Availability: |
https://doi.org/10.3390/ijms26031272 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.1644E911 |
| Database: |
BASE |