| Title: |
Cloning and analysis of the gene for the major outer membrane lipoprotein from Pseudomonas aeruginosa |
| Authors: |
Cornelis, P.; Bouia, A.; Belarbi, A.; Guyonvarch, A.; Kammerer, B.; Hannaert, V.; Hubert, J. C. |
| Source: |
Molecular Microbiology ; volume 3, issue 3, page 421-428 ; ISSN 0950-382X 1365-2958 |
| Publisher Information: |
Wiley |
| Publication Year: |
1989 |
| Collection: |
Wiley Online Library (Open Access Articles via Crossref) |
| Description: |
Summary The gene for the Pseudomonas aeruginosa outer membrane lipoprotein I was isolated from a genomic library in the phage λ EMBL3 vector and subsequently subcloned in the low copy‐number, wide host‐range plasmid vector, pKT240. The cloned gene was highly expressed, resulting in the production of a low molecular‐weight protein (8kD) that was found to be associated with the outer membrane. Sequence anatysis showed an open reading frame of 83 amino acids with a putative N‐terminal hydrophobic signal peptide of 19 residues immediately followed by the lipoprotein consensus sequence, GLY‐CYS‐SER‐SER (residues 19–22). The predicted amino acid composition of the mature polypeptide and that of the purified lipoprotein I of P. aeruginosa (Mizuno and Kageyama, 1979) were identical. In contrast with other Gram‐negative outer membrane lipoproteins, conformation predictions suggested that the mature protein was a single alpha helix. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1111/j.1365-2958.1989.tb00187.x |
| Availability: |
https://doi.org/10.1111/j.1365-2958.1989.tb00187.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2958.1989.tb00187.x; https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2958.1989.tb00187.x |
| Rights: |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
| Accession Number: |
edsbas.16944FEB |
| Database: |
BASE |