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Soil extracellular enzymes drive soil carbon accumulation under elevated CO 2

Title: Soil extracellular enzymes drive soil carbon accumulation under elevated CO 2
Authors: Zhang, Yixuan; Sun, Siyi; Zhou, Jiacong; van Groenigen, Kees Jan; Delgado-Baquerizo, Manuel; Ma, Ying; Moorhead, Daryl L.; Hungate, Bruce A.; Smith, Pete; Terrer, César; Liu, Ji; Sinsabaugh, Robert L.; Guo, Liping; Ochoa-Hueso, Raúl; Power, Sally A.; Eivind Olesen, Jørgen; Luo, Yiqi; Cao, Junji; Jiang, Mingkai; Feng, Zhaozhong; Luo, Min; Chen, Ji
Source: Zhang, Y, Sun, S, Zhou, J, van Groenigen, K J, Delgado-Baquerizo, M, Ma, Y, Moorhead, D L, Hungate, B A, Smith, P, Terrer, C, Liu, J, Sinsabaugh, R L, Guo, L, Ochoa-Hueso, R, Power, S A, Eivind Olesen, J, Luo, Y, Cao, J, Jiang, M, Feng, Z, Luo, M & Chen, J 2026, 'Soil extracellular enzymes drive soil carbon accumulation under elevated CO 2', Functional Ecology, vol. 40, no. 2, pp. 347-359. https://doi.org/10.1111/1365-2435.70249
Publication Year: 2026
Collection: Aarhus University: Research
Subject Terms: carbon-climate feedback; elevated CO; soil extracellular enzyme; soil microorganism; soil nutrient; soil organic carbon
Description: Human-driven increases in atmospheric CO 2 (eCO 2 ) are stimulating plant growth, thereby increasing the input of plant-derived carbon into soils. The fate of this additional carbon depends on the capacity of soil microbiomes to decompose and transform organic matter, a central process in regulating soil organic carbon (SOC) dynamics. However, how eCO 2 affects this microbial capacity remains poorly understood. Because soil extracellular enzymes catalyse the degradation of various SOC pools, their activities (extracellular enzyme activities, EEAs) could offer mechanistic insights into microbially mediated SOC dynamics. We synthesized 272 observations on SOC and EEAs from eCO 2 experiments across farmland, forest, grassland and shrubland, combining classical meta-analysis with random forest modelling. Our results showed that eCO 2 significantly increased SOC by 4.2%. Among all variables tested, increased cellulase activity, which targets the breakdown of labile carbon sources, emerged as the strongest predictor of SOC accumulation. Specifically, eCO 2 stimulated cellulase activity by 12.2% but had no effect on ligninase activity, which decomposes recalcitrant carbon. This enzymatic shift was likely driven by increased plant-derived labile carbon inputs under eCO 2 and was associated with changes in the soil microbiome, including a higher fungi-to-bacteria ratio. These results underscore the potential of EEA as a predictive indicator of SOC accumulation under eCO 2 and the importance of representing enzymatic processes in Earth system models. Read the free Plain Language Summary for this article on the Journal blog.
Document Type: article in journal/newspaper
Language: English
ISSN: 0269-8463; 1365-2435
Relation: info:eu-repo/semantics/altIdentifier/pissn/0269-8463; info:eu-repo/semantics/altIdentifier/eissn/1365-2435
DOI: 10.1111/1365-2435.70249
Availability: https://pure.au.dk/portal/en/publications/442ee6ef-2999-47fe-8f05-5952ebfc1627; https://doi.org/10.1111/1365-2435.70249; https://www.scopus.com/pages/publications/105026370254
Rights: info:eu-repo/semantics/openAccess
Accession Number: edsbas.175DBA08
Database: BASE