| Title: |
Gut Microbial Similarity Analysis in Mono and Dizygotic Twins Discordant for Down Syndrome |
| Authors: |
Maillard, Pierre-Yves; Tokajian, Sima; Vilaire-Meunier, Marie; Khoury, Charbel Al; Mehawej, Cybel; Chouery, Eliane; Caillaud, Marie-Anne; Maillebouis, Louise; Smida, Ines Ben; Durand, Sophie; Ravel, Aimé; Cieuta-Walti, Cecile; Ungeheuer, Marie-Noëlle; Mircher, Clotilde; Mégarbané, André |
| Source: |
OBM Genetics ; volume 09, issue 03, page 1-22 ; ISSN 2577-5790 |
| Publisher Information: |
LIDSEN Publishing Inc |
| Publication Year: |
2025 |
| Description: |
To investigate the potential impact of the additional chromosome 21 on the gut microbiome in patients with Down syndrome (DS), two monozygotic (MZ) and two dizygotic (DZ) twin pairs discordant for DS were studied. Whole-genome shotgun sequencing was conducted to analyze the taxonomic and functional profiles of the gut microbial community in the twins and assess whether the gut microbiome composition influences the development of psychological pathologies. Sequencing generated a total of 2,338,132 reads, combined across the four samples. The number of contigs ranged from 98,521 to 122,000, and the GC content was 46.34-47.15%. The Simpson index at the species level in the DS of our study was distinctly higher in the DZ-DS but not in the MZ-DS. Taxonomic classification revealed a Western diversity profile in the twins, where the dominant bacterial phyla were Firmicutes, Bacteroidetes, and Actinomycetota. Each sample had a diversity of ten families at abundance >0.5%, of which Prevotellaceae, Lachnospiraceae, and Bacteroidaceae were the most prevalent. The Prevotellaceae family was detected at a high level in all samples, except in the DZ-DS, where Lachnospiraceae was the most abundant family. At the genus level, marked differences were observed between the DZ-DS twins compared to the others. Prevotella was not detected in the DZ-DS twin whereas it was the dominant genus in the MZ-DS and both healthy twins. The variation in predicted metagenomes between DS and non-DS showed that the average relative abundances for DS were comparable to those for non-DS. The two MZ twins discordant for DS appear to have relatively similar microbiomes, suggesting that the added chromosome 21 did not have a significant impact on gut microbiome composition. Identifying biomarkers of DS that modulate the gut composition and affect the overall health needs to be further studied, along with the potential role of Prevotella. |
| Document Type: |
article in journal/newspaper |
| Language: |
unknown |
| DOI: |
10.21926/obm.genet.2503300 |
| Availability: |
https://doi.org/10.21926/obm.genet.2503300; https://www.lidsen.com/journals/genetics/genetics-09-03-300/obm.genet.2503300.xml; https://www.lidsen.com/journals/genetics/genetics-09-03-300 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.180DFA8E |
| Database: |
BASE |