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Role of Ovalbumin/β-Cyclodextrin in Improving Structural and Gelling Properties of Culter alburnus Myofibrillar Proteins during Frozen Storage

Title: Role of Ovalbumin/β-Cyclodextrin in Improving Structural and Gelling Properties of Culter alburnus Myofibrillar Proteins during Frozen Storage
Authors: Meiwen Lv; Xiukang Wang; Noman Walayat; Zhongli Zhang; Muhammad Hamzah Saleem; Asad Nawaz; Rana Muhammad Aadil; Shakeel Ahmed; Mario J. Simirgiotis; José M. Lorenzo; Basit Latief Jan
Source: Applied Sciences ; Volume 11 ; Issue 24 ; Pages: 11815
Publisher Information: Multidisciplinary Digital Publishing Institute
Publication Year: 2021
Collection: MDPI Open Access Publishing
Subject Terms: myofibrillar proteins; ovalbumin; β-cyclodextrin; cryoprotectant; frozen storage
Description: This study aimed to analyze the cryoprotective effect of a ovalbumin (OVA) and β-cyclodextrin (βCD) mixture (3:1, OVA/βCD) on the structure, rheology and gelling properties of myofibrillar proteins (MPs) during 90 days of frozen storage. A mixture of OVA/βCD at different concentrations (0, 2, 4, and 6%) was added to MPs and stored at −18 °C for 90 days. The addition of OVA/βCD significantly decreased the sulfhydryl contents while it increased the surface hydrophobicity, which was closely connected with tertiary structural changes. Circular dichroism analysis showed that the addition of OVA/βCD enhanced the stability of the secondary structure by inhibiting the decline in the α-helix. Rheological properties analysis indicated that 6% OVA/βCD treatment showed better storage modulus (G’) and loss modulus (G”). In addition, treatment of OVA/βCD showed better gel forming properties than the control group (0%), helping to form a homogeneous and denser gel network. The results proved that 6% OVA/βCD could be act as a promising cryoprotectant, which can improve the structure and gel behavior of Culter alburnus MPs during frozen storage. Moreover, OVA/βCD could be a potential alternative to conventional cryoprotectants at the industrial level to increase the economic and commercial values of seafood products.
Document Type: text
File Description: application/pdf
Language: English
Relation: Food Science and Technology; https://dx.doi.org/10.3390/app112411815
DOI: 10.3390/app112411815
Availability: https://doi.org/10.3390/app112411815
Rights: https://creativecommons.org/licenses/by/4.0/
Accession Number: edsbas.18C01E49
Database: BASE