| Title: |
Characterization of ESBL (SHV-12) producing clinical isolate of Enterobacter aerogenes from a tertiary care hospital in Nigeria ; Annals of Clinical Microbiology and Antimicrobials |
| Authors: |
Kasap, Murat; Fashae, Kayode; Torol, Sinem; Kolayli, Fetiye; Budak, Fatma; Vahaboglu, Haluk |
| Publication Year: |
2010 |
| Collection: |
VTechWorks (VirginiaTech) |
| Description: |
Background We studied the beta-lactamases of an E. aerogenes isolate recovered from the blood of a two-year-old patient. The isolate demonstrated a disk-diffusion phenotype typical for an AmpC-ESBL co-producer. Methods Microbiology studies were performed according to standard protocols. The resistance gene was identified by transconjugation and cloning experiments. Results By transconjugation only a narrow spectrum beta-lactamase (TEM-1) encoded on a small plasmid was transmitted. The ESBL was cloned and expressed in an E. coli host. Sequence analysis of the recombinant plasmid revealed blaSHV-12 associated to the insertion sequence, IS26. Conclusion This is the first study demonstrated the occurrence of SHV-12 in Nigeria. ; Published version |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
Annals of Clinical Microbiology and Antimicrobials. 2010 Jan 12;9(1):1; https://hdl.handle.net/10919/18850 |
| DOI: |
10.1186/1476-0711-9-1 |
| Availability: |
https://hdl.handle.net/10919/18850; https://doi.org/10.1186/1476-0711-9-1 |
| Rights: |
Creative Commons Attribution 4.0 International ; http://creativecommons.org/licenses/by/4.0/ ; Murat Kasap et al.; licensee BioMed Central Ltd. |
| Accession Number: |
edsbas.1A325DD6 |
| Database: |
BASE |