| Title: |
A single bout of resistance exercise triggers mitophagy, potentially involving the ejection of mitochondria in human skeletal muscle. |
| Authors: |
Díaz-Castro, F.; Tuñón-Suárez, M.; Rivera, P.; Botella, J.; Cancino, J.; Figueroa, A.M.; Gutiérrez, J.; Cantin, C.; Deldicque, L.; Zbinden-Foncea, H.; Nielsen, J.; Henríquez-Olguín, C.; Morselli, E.; Castro-Sepúlveda, M. |
| Publication Year: |
2024 |
| Collection: |
Université de Lausanne (UNIL): Serval - Serveur académique lausannois |
| Subject Terms: |
Humans; Mitophagy/physiology; Male; Muscle; Skeletal/metabolism; Skeletal/physiology; Adult; Mitochondria; Muscle/metabolism; Muscle/ultrastructure; Resistance Training; Young Adult; Membrane Proteins/metabolism; BNIP3L/NIX; mitochondria cristae; mitochondria dynamics; mitophagy |
| Description: |
The present study aimed to investigate the effects of a single bout of resistance exercise on mitophagy in human skeletal muscle (SkM). Eight healthy men were recruited to complete an acute bout of one-leg resistance exercise. SkM biopsies were obtained one hour after exercise in the resting leg (Rest-leg) and the contracting leg (Ex-leg). Mitophagy was assessed using protein-related abundance, transmission electron microscopy (TEM), and fluorescence microscopy. Our results show that acute resistance exercise increased pro-fission protein phosphorylation (DRP1 Ser616 ) and decreased mitophagy markers such as PARKIN and BNIP3L/NIX protein abundance in the Ex-leg. Additionally, mitochondrial complex IV decreased in the Ex-leg when compared to the Rest-leg. In the Ex-leg, TEM and immunofluorescence images showed mitochondrial cristae abnormalities, a mitochondrial fission phenotype, and increased mitophagosome-like structures in both subsarcolemmal and intermyofibrillar mitochondria. We also observed increased mitophagosome-like structures on the subsarcolemmal cleft and mitochondria in the extracellular space of SkM in the Ex-leg. We stimulated human primary myotubes with CCCP, which mimics mitophagy induction in the Ex-leg, and found that BNIP3L/NIX protein abundance decreased independently of lysosomal degradation. Finally, in another human cohort, we found a negative association between BNIP3L/NIX protein abundance with both mitophagosome-like structures and mitochondrial cristae density in the SkM. The findings suggest that a single bout of resistance exercise can initiate mitophagy, potentially involving mitochondrial ejection, in human skeletal muscle. BNIP3L/NIX is proposed as a sensitive marker for assessing mitophagy flux in SkM. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| ISSN: |
1748-1716 |
| Relation: |
Acta Physiologica; https://iris.unil.ch/handle/iris/134573; serval:BIB_B35E10FCF638; 001270555600001 |
| DOI: |
10.1111/apha.14203 |
| Availability: |
https://iris.unil.ch/handle/iris/134573; https://doi.org/10.1111/apha.14203 |
| Accession Number: |
edsbas.1B670E41 |
| Database: |
BASE |