| Title: |
Scalable and High-Throughput In Vitro Vibratory Platform for Vocal Fold Tissue Engineering Applications |
| Authors: |
Andreea Biehl; Ramair Colmon; Anastasia Timofeeva; Ana Maria Gracioso Martins; Gregory R. Dion; Kara Peters; Donald O. Freytes |
| Source: |
Bioengineering ; Volume 10 ; Issue 5 ; Pages: 602 |
| Publisher Information: |
Multidisciplinary Digital Publishing Institute |
| Publication Year: |
2023 |
| Collection: |
MDPI Open Access Publishing |
| Subject Terms: |
vocal fold; bioreactor; piezoelectric speaker; vibration; frequency; displacement; fibroblasts; mesenchymal stem cells; gene expression |
| Description: |
The vocal folds (VFs) are constantly exposed to mechanical stimulation leading to changes in biomechanical properties, structure, and composition. The development of long-term strategies for VF treatment depends on the characterization of related cells, biomaterials, or engineered tissues in a controlled mechanical environment. Our aim was to design, develop, and characterize a scalable and high-throughput platform that mimics the mechanical microenvironment of the VFs in vitro. The platform consists of a 24-well plate fitted with a flexible membrane atop a waveguide equipped with piezoelectric speakers which allows for cells to be exposed to various phonatory stimuli. The displacements of the flexible membrane were characterized via Laser Doppler Vibrometry (LDV). Human VF fibroblasts and mesenchymal stem cells were seeded, exposed to various vibratory regimes, and the expression of pro-fibrotic and pro-inflammatory genes was analyzed. Compared to current bioreactor designs, the platform developed in this study can incorporate commercial assay formats ranging from 6- to 96-well plates which represents a significant improvement in scalability. This platform is modular and allows for tunable frequency regimes. |
| Document Type: |
text |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
https://dx.doi.org/10.3390/bioengineering10050602 |
| DOI: |
10.3390/bioengineering10050602 |
| Availability: |
https://doi.org/10.3390/bioengineering10050602 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.1BB5601D |
| Database: |
BASE |