| Title: |
Single-cell multi-omics analysis of the immune response in COVID-19 |
| Authors: |
Stephenson, E; Reynolds, G; Botting, RA; Calero-Nieto, FJ; Morgan, MD; Tuong, ZK; Bach, K; Sungnak, W; Worlock, KB; Yoshida, M; Kumasaka, N; Kania, K; Engelbert, J; Olabi, B; Spegarova, JS; Wilson, NK; Mende, N; Jardine, L; Gardner, LCS; Goh, I; Horsfall, D; McGrath, J; Webb, S; Mather, MW; Lindeboom, RGH; Dann, E; Huang, N; Polanski, K; Prigmore, E; Gothe, F; Scott, J; Payne, RP; Baker, KF; Hanrath, AT; Schim van der Loeff, ICD; Barr, AS; Sanchez-Gonzalez, A; Bergamaschi, L; Mescia, F; Barnes, JL; Kilich, E; de Wilton, A; Saigal, A; Saleh, A; Janes, SM; Smith, CM; Gopee, N; Wilson, C; Coupland, P; Coxhead, JM; Kiselev, VY; van Dongen, S; Bacardit, J; King, HW; Baker, S; Bradley, JR; Dougan, G; Goodfellow, IG; Gupta, RK; Hess, C; Kingston, N; Lehner, PJ; Matheson, NJ; Owehand, WH; Saunders, C; Smith, KGC; Summers, C; Thaventhiran, JED; Toshner, M; Weekes, MP; Bucke, A; Calder, J; Canna, L; Domingo, J; Elmer, A; Fuller, S; Harris, J; Hewitt, S; Kennet, J; Jose, S; Kourampa, J; Meadows, A; O’Brien, C; Price, J; Publico, C; Rastall, R; Ribeiro, C; Rowlands, J; Ruffolo, V; Tordesillas, H; Bullman, B; Dunmore, BJ; Fawke, S; Gräf, S; Hodgson, J; Huang, C; Hunter, K; Jones, E; Legchenko, E; Matara, C |
| Publisher Information: |
NATURE PORTFOLIO |
| Publication Year: |
2021 |
| Collection: |
The University of Melbourne: Digital Repository |
| Description: |
Analysis of human blood immune cells provides insights into the coordinated response to viral infections such as severe acute respiratory syndrome coronavirus 2, which causes coronavirus disease 2019 (COVID-19). We performed single-cell transcriptome, surface proteome and T and B lymphocyte antigen receptor analyses of over 780,000 peripheral blood mononuclear cells from a cross-sectional cohort of 130 patients with varying severities of COVID-19. We identified expansion of nonclassical monocytes expressing complement transcripts (CD16+C1QA/B/C+) that sequester platelets and were predicted to replenish the alveolar macrophage pool in COVID-19. Early, uncommitted CD34+ hematopoietic stem/progenitor cells were primed toward megakaryopoiesis, accompanied by expanded megakaryocyte-committed progenitors and increased platelet activation. Clonally expanded CD8+ T cells and an increased ratio of CD8+ effector T cells to effector memory T cells characterized severe disease, while circulating follicular helper T cells accompanied mild disease. We observed a relative loss of IgA2 in symptomatic disease despite an overall expansion of plasmablasts and plasma cells. Our study highlights the coordinated immune response that contributes to COVID-19 pathogenesis and reveals discrete cellular components that can be targeted for therapy. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| ISSN: |
1078-8956 |
| Relation: |
https://hdl.handle.net/11343/309136 |
| Availability: |
https://hdl.handle.net/11343/309136 |
| Rights: |
https://creativecommons.org/licenses/by/4.0 ; CC BY |
| Accession Number: |
edsbas.1BB807A7 |
| Database: |
BASE |