| Title: |
Insulin-transferrin-selenium as an alternative to foetal serum for epidermal equivalents |
| Authors: |
Mainzer, C.; Barrichello, C.; Debret, R.; Remoué, N.; Sigaudo-Roussel, D.; Sommer, P. |
| Contributors: |
Matrice extracellulaire et régulations cellulaires (MERC); Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS); Institut de biologie et chimie des protéines Lyon (IBCP); Université Claude Bernard Lyon 1 (UCBL); Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS) |
| Source: |
ISSN: 0142-5463. |
| Publisher Information: |
CCSD; Wiley |
| Publication Year: |
2014 |
| Collection: |
Université de Reims Champagne-Ardenne: Archives Ouvertes (HAL) |
| Subject Terms: |
skin physiology/structure; serum free; safety testing; insulin-transferrin-selenium; epidermal equivalent; cell culture; [SDV]Life Sciences [q-bio] |
| Description: |
International audience ; Organotypic skin models are powerful tools for research in development, ageing and diseases. They have become more and more complex with the use of multiple cell types. This requires a culture medium adapted to optimize the development of such in vitro skin. Foetal bovine serum (FBS) is the most complete supplement in existence at the moment, providing at once growth factors, vitamins, hormones and other circulating compounds. However, this cocktail suffers from batch variability and its animal origin is ethically questionable. More importantly, its biological activities may interfere with the study of certain signalling pathways. Here, we present a strategy for constructing an epidermal equivalent using a defined culture medium without serum. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/24847782; PUBMED: 24847782 |
| DOI: |
10.1111/ics.12141 |
| Availability: |
https://hal.science/hal-02340139; https://doi.org/10.1111/ics.12141 |
| Accession Number: |
edsbas.1C530E82 |
| Database: |
BASE |