| Title: |
Mice prenatally exposed to valproic acid do not show autism-related disorders when fed with polyunsaturated fatty acid-enriched diets |
| Authors: |
Turpin, Valentine; Schaffhauser, Maud; Thabault, Mathieu; Aubert, Agnès; Joffre, Corinne; Balado, Eric; Longueville, Jean-Emmanuel; Francheteau, Maureen; Burucoa, Christophe; Pichon, Maxime; Layé, Sophie; Jaber, Mohamed |
| Contributors: |
Laboratoire de neurosciences expérimentales et cliniques U 1084 (LNEC Poitiers ); Université de Poitiers = University of Poitiers (UP)-Institut National de la Santé et de la Recherche Médicale (INSERM); Nutrition et Neurobiologie intégrée (NutriNeuro); Université de Bordeaux (UB)-Institut Polytechnique de Bordeaux-Ecole nationale supérieure de chimie, biologie et physique-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); Pharmacologie des anti-infectieux et antibiorésistance U 1070 (PHAR2 Poitiers ); Centre hospitalier universitaire de Poitiers = Poitiers University Hospital (CHU de Poitiers La Milétrie ); Université de Poitiers – UFR Santé Faculté de Médecine et de Pharmacie (UFR Santé Poitiers ); Université de Poitiers = University of Poitiers (UP); The research leading to the results detailed in this manuscript received funding from the Fondation pour la Recherche Medicale (MJ) and the CPER-FEDER program (MJ), FRC CONNECT (SL) and Region Nouvelle Aquitaine (SL and MJ, 2019-1R3M08). VT is a recipient of a joint PhD fellowship from Nouvelle-Aquitaine Region/Fondation pour la Recherche Medicale. MT is a recipient of a joint PhD fellowship from Nouvelle-Aquitaine Region/Inserm. This work has benefited from the facilities and expertise of the PREBIOS platform (Universite de Poitiers). Authors are grateful for the research staff at the Eye and Nutrition Research Group for lipidomic analyses (UMR1324 INRAE, Dijon, France). Authors are thankful to Jeffrey Arsham and Nathaniel L. Ritz for careful language editing. Some of these results were recently presented in a poster format at the FENS meeting in Paris (July 2022). |
| Source: |
ISSN: 2045-2322. |
| Publisher Information: |
CCSD; Nature Publishing Group |
| Publication Year: |
2023 |
| Collection: |
Université de Poitiers: Publications de nos chercheurs.ses (HAL) |
| Subject Terms: |
maternal-care; inflammation; supplementation; omega-3-fatty-acids; pufas; behavior; brain; mouse model; gut microbiota; spectrum disorders; MESH: Female; MESH: Male; MESH: Fatty Acids; Unsaturated; Omega-3; MESH: Dietary Supplements; MESH: Animals; MESH: Mice; MESH: Autistic Disorder; MESH: Autism Spectrum Disorder; MESH: Valproic Acid; MESH: Diet; [SCCO.NEUR]Cognitive science/Neuroscience |
| Description: |
International audience ; Dietary supplementations with n-3 polyunsaturated fatty acid (PUFA) have been explored in autism spectrum disorder (ASD) but their efficiency and potential in ameliorating cardinal symptoms of the disease remain elusive. Here, we compared a n-3 long-chain (LC) PUFA dietary supplementation (n-3 supp) obtained from fatty fish with a n-3 PUFA precursor diet (n-3 bal) obtained from plant oils in the valproic acid (VPA, 450 mg/kg at E12.5) ASD mouse model starting from embryonic life, throughout lactation and until adulthood. Maternal and offspring behaviors were investigated as well as several VPA-induced ASD biological features: cerebellar Purkinje cell (PC) number, inflammatory markers, gut microbiota, and peripheral and brain PUFA composition. Developmental milestones were delayed in the n-3 supp group compared to the n-3 bal group in both sexes. Whatever the diet, VPA-exposed offspring did not show ASD characteristic alterations in social behavior, stereotypies, PC number, or gut microbiota dysbiosis while global activity, gait, peripheral and brain PUFA levels as well as cerebellar TNF-alpha levels were differentially altered by diet and treatment according to sex. The current study provides evidence of beneficial effects of n-3 PUFA based diets, including one without LCPUFAs, on preventing several behavioral and cellular symptoms related to ASD. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/37433863; PUBMED: 37433863; PUBMEDCENTRAL: PMC10336048 |
| DOI: |
10.1038/s41598-023-38423-z |
| Availability: |
https://hal.science/hal-05020816; https://doi.org/10.1038/s41598-023-38423-z |
| Accession Number: |
edsbas.1E6486DC |
| Database: |
BASE |