| Title: |
Comparative transcriptomics reveals striking similarities between the bovine and feline isolates of Tritrichomonas foetus : consequences for in silico drug-target identification |
| Authors: |
Morin-Adeline, Victoria; Lomas, Rodrigo; O'Mealy, Denis; Stack, Colin M. (R12090); Conesa, Ana; Slapeta, Jan |
| Contributors: |
School of Science and Health (Host institution) |
| Publisher Information: |
U.K., BioMed Central |
| Publication Year: |
2014 |
| Collection: |
University of Western Sydney (UWS): Research Direct |
| Subject Terms: |
060502 - Infectious Agents; 110309 - Infectious Diseases; 060503 - Microbial Genetics; 860901 - Veterinary Biological Preventatives (e.g. Vaccines) |
| Description: |
Background : Few, if any, protozoan parasites are reported to exhibit extreme organ tropism like the flagellate Tritrichomonas foetus. In cattle, T. foetus infects the reproductive system causing abortion, whereas the infection in cats results in chronic large bowel diarrhoea. In the absence of a T. foetus genome, we utilized a de novo approach to assemble the transcriptome of the bovine and feline genotype to identify host-specific adaptations and virulence factors specific to each genotype. Furthermore, a subset of orthologs was used to characterize putative druggable targets and expose complications of in silico drug target mining in species with indefinite host-ranges. Results - Illumina RNA-seq reads were assembled into two representative bovine and feline transcriptomes containing 42,363 and 36,559 contigs, respectively. Coding and non-coding regions of the genome libraries revealed striking similarities, with 24,620 shared homolog pairs reduced down to 7,547 coding orthologs between the two genotypes. The transcriptomes were near identical in functional category distribution; with no indication of selective pressure acting on orthologs despite differences in parasite origins/host. Orthologs formed a large proportion of highly expressed transcripts in both genotypes (bovine genotype: 76%, feline genotype: 56%). Mining the libraries for protease virulence factors revealed the cysteine proteases (CP) to be the most common. In total, 483 and 445 bovine and feline T. foetus transcripts were identified as putative proteases based on MEROPS database, with 9 hits to putative protease inhibitors. In bovine T. foetus, CP8 is the preferentially transcribed CP while in the feline genotype, transcription of CP7 showed higher abundance. In silico druggability analysis of the two genotypes revealed that when host sequences are taken into account, drug targets are genotype-specific. Conclusion - Gene discovery analysis based on RNA-seq data analysis revealed prominent similarities between the bovine and feline T. foetus, ... |
| Document Type: |
article in journal/newspaper |
| File Description: |
print |
| Language: |
English |
| Relation: |
BMC Genomics--1471-2164--1471-2164 Vol. 15 Issue. 955 |
| DOI: |
10.1186/1471-2164-15-955 |
| Availability: |
http://handle.uws.edu.au:8081/1959.7/556341; https://doi.org/10.1186/1471-2164-15-955 |
| Rights: |
© 2014 Morin-Adeline et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
| Accession Number: |
edsbas.1EC4050C |
| Database: |
BASE |