| Title: |
Expression Analysis of the Stem Cell Marker Pw1/Peg3 Reveals a CD34 Negative Progenitor Population in the Hair Follicle |
| Authors: |
Besson, Vanessa; Kyryachenko, Sergiy; Janich, Peggy; Benitah, Salvador A.; Marazzi, Giovanna; Sassoon, David |
| Contributors: |
Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Research Unit on Cardiovascular and Metabolic Diseases (ICAN); Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière AP-HP; Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU) |
| Source: |
Stem Cells ; https://hal.science/hal-01515292 ; Stem Cells, 2017, 35 (4), pp.1015-1027. ⟨10.1002/stem.2540⟩ |
| Publisher Information: |
CCSD |
| Publication Year: |
2017 |
| Subject Terms: |
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry; Molecular Biology/Genomics [q-bio.GN] |
| Description: |
International audience ; Pw1/Peg3 is a parentally imprinted gene expressed in adult stem cells in every tissue thus far examined including the stem cells of the hair follicle. Using a Pw1/Peg3 reporter mouse, we carried out a detailed dissection of the stem cells in the bulge, which is a major stem cell compartment of the hair follicle in mammalian skin. We observed that PW1/Peg3 expression initiates upon placode formation during fetal development, coincident with the establishment of the bulge stem cells. In the adult, we observed that PW1/Peg3 expression is found in both CD34+ and CD34- populations of bulge stem cells. We demonstrate that both populations can give rise to new hair follicles, reconstitute their niche, and self-renew. These results demonstrate that PW1/Peg3 is a reliable marker of the full population of follicle stem cells and reveal a novel CD34- bulge stem-cell population. Stem Cells 2017;35:1015–1027 |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1002/stem.2540 |
| Availability: |
https://hal.science/hal-01515292; https://doi.org/10.1002/stem.2540 |
| Accession Number: |
edsbas.1EFE1E0B |
| Database: |
BASE |