| Title: |
The DNA-binding network of Mycobacterium tuberculosi s |
| Authors: |
Minch, Kyle J.; Rustad, Tige R.; Peterson, Eliza J. R.; Winkler, Jessica; Reiss, David J.; Ma, Shuyi; Hickey, Mark; Brabant, William; Morrison, Bob; Turkarslan, Serdar; Mawhinney, Chris; Galagan, James E.; Price, Nathan D.; Baliga, Nitin S.; Sherman, David R. |
| Source: |
Nature Communications ; volume 6, issue 1 ; ISSN 2041-1723 |
| Publisher Information: |
Springer Science and Business Media LLC |
| Publication Year: |
2015 |
| Description: |
Mycobacterium tuberculosis (MTB) infects 30% of all humans and kills someone every 20–30 s. Here we report genome-wide binding for ~80% of all predicted MTB transcription factors (TFs), and assayed global expression following induction of each TF. The MTB DNA-binding network consists of ~16,000 binding events from 154 TFs. We identify >50 TF-DNA consensus motifs and >1,150 promoter-binding events directly associated with proximal gene regulation. An additional ~4,200 binding events are in promoter windows and represent strong candidates for direct transcriptional regulation under appropriate environmental conditions. However, we also identify >10,000 ‘dormant’ DNA-binding events that cannot be linked directly with proximal transcriptional control, suggesting that widespread DNA binding may be a common feature that should be considered when developing global models of coordinated gene expression. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1038/ncomms6829 |
| Availability: |
https://doi.org/10.1038/ncomms6829; https://www.nature.com/articles/ncomms6829.pdf; https://www.nature.com/articles/ncomms6829 |
| Rights: |
https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| Accession Number: |
edsbas.2005A6D |
| Database: |
BASE |