Loss of centromere function drives karyotype evolution in closely related Malassezia species
| Title: | Loss of centromere function drives karyotype evolution in closely related Malassezia species |
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| Authors: | Sankaranarayanan, Sundar Ram; Ianiri, Giuseppe; Coelho, Marco A.; Reza, Md Hashim; Thimmappa, Bhagya C.; Ganguly, Promit; Vadnala, Rakesh Netha; Sun, Sheng; Siddharthan, Rahul; Tellgren-Roth, Christian; Dawson, Thomas L. Jnr; Heitman, Joseph; Sanyal, Kaustuv |
| Publisher Information: | Uppsala universitet, Institutionen för immunologi, genetik och patologi; Uppsala universitet, Science for Life Laboratory, SciLifeLab; Jawaharlal Nehru Ctr Adv Sci Res, Mol Mycol Lab, Mol Biol & Genet Unit, Bengaluru, India; Duke Univ, Med Ctr, Dept Mol Genet & Microbiol, Durham, NC 27710 USA;Univ Molise, Dept Agr Environm & Food Sci, Campobasso, Italy; Duke Univ, Med Ctr, Dept Mol Genet & Microbiol, Durham, NC 27710 USA; Jawaharlal Nehru Ctr Adv Sci Res, Mol Mycol Lab, Mol Biol & Genet Unit, Bengaluru, India;Univ Montreal, Robert Cedergren Ctr Bioinformat & Genom, Dept Biochem, Montreal, PQ, Canada; Inst Math Sci HBNI, Chennai, Tamil Nadu, India; ASTAR, Skin Res Inst Singapore, Singapore, Singapore;Med Univ South Carolina, Dept Drug Discovery, Charleston, SC 29425 USA; ELIFE SCIENCES PUBLICATIONS LTD |
| Publication Year: | 2020 |
| Collection: | Uppsala University: Publications (DiVA) |
| Subject Terms: | Genetics and Genomics; Genetik och genomik |
| Description: | Genomic rearrangements associated with speciation often result in variation in chromosome number among closely related species. Malassezia species show variable karyotypes ranging between six and nine chromosomes. Here, we experimentally identified all eight centromeres in M. sympodialis as 3-5-kb long kinetochore-bound regions that span an AT-rich core and are depleted of the canonical histone H3. Centromeres of similar sequence features were identified as CENP-A-rich regions in Malassezia furfur, which has seven chromosomes, and histone H3 depleted regions in Malassezia slooffiae and Malassezia globosa with nine chromosomes each. Analysis of synteny conservation across centromeres with newly generated chromosome-level genome assemblies suggests two distinct mechanisms of chromosome number reduction from an inferred nine-chromosome ancestral state: (a) chromosome breakage followed by loss of centromere DNA and (b) centromere inactivation accompanied by changes in DNA sequence following chromosome-chromosome fusion. We propose that AT-rich centromeres drive karyotype diversity in the Malassezia species complex through breakage and inactivation. |
| Document Type: | article in journal/newspaper |
| File Description: | application/pdf |
| Language: | English |
| Relation: | eLIFE, 2020, 9; PMID 31958060; ISI:000515163700001 |
| DOI: | 10.7554/eLife.53944 |
| Availability: | http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-407644; https://doi.org/10.7554/eLife.53944 |
| Rights: | info:eu-repo/semantics/openAccess |
| Accession Number: | edsbas.21346C83 |
| Database: | BASE |