| Title: |
Continual Conscious Bioluminescent Imaging in Freely Moving Mice |
| Authors: |
Diaz, JA; Geard, A; FitzPatrick, LM; Delhove, JMKM; Buckley, SMK; Waddington, SN; McKay, TR; Karda, R |
| Source: |
Methods in Molecular Biology , 2081 pp. 161-175. (2019) |
| Publication Year: |
2019 |
| Collection: |
University College London: UCL Discovery |
| Subject Terms: |
Lentiviral vectors; Adeno-associated vectors; Transcription factor activated reporter; Biosensors; Luciferase; Neonatal mice; Luciferin; Bioluminescence imaging; Conscious imaging |
| Description: |
In vivo bioluminescent imaging allows the detection of reporter gene expression in rodents in real time. Here we describe a novel technology whereby we can generate somatotransgenic rodents with the use of a viral vector carrying a luciferase transgene. We are able to achieve long term luciferase expression by a single injection of lentiviral or adeno-associated virus vectors to newborn mice. Further, we describe whole body bioluminescence imaging of conscious mice in a noninvasive manner, thus enforcing the 3R’s (replacement, reduction, and refinement) of biomedical animal research. |
| Document Type: |
article in journal/newspaper |
| File Description: |
text |
| Language: |
English |
| Relation: |
https://discovery.ucl.ac.uk/id/eprint/10090105/1/Continual%20Conscious%20Bioluminescent%20Imaging%20in%20Freely%20Moving%20Mice.%20Methods%20Mol%20Biol%20,%202081%20pp.pdf; https://discovery.ucl.ac.uk/id/eprint/10090105/ |
| Availability: |
https://discovery.ucl.ac.uk/id/eprint/10090105/1/Continual%20Conscious%20Bioluminescent%20Imaging%20in%20Freely%20Moving%20Mice.%20Methods%20Mol%20Biol%20,%202081%20pp.pdf; https://discovery.ucl.ac.uk/id/eprint/10090105/ |
| Rights: |
open |
| Accession Number: |
edsbas.22654B62 |
| Database: |
BASE |