| Title: |
Introducing affinity and selectivity into galectin-targeting nanoparticles with fluorinated glycan ligands |
| Authors: |
Richards, S-J; Keenan, T; Vendeville, J-B; Wheatley, DE; Chidwick, H; Budhadev, D; Council, CE; Webster, CS; Ledru, H; Baker, AN; Walker, M; Galan, MC; Linclau, B; Fascione, MA; Gibson, MI |
| Publisher Information: |
Royal Society of Chemistry (RSC) |
| Publication Year: |
2021 |
| Collection: |
White Rose Research Online (Universities of Leeds, Sheffield & York) |
| Description: |
Galectins are potential biomarkers and therapeutic targets. However, galectins display broad affinity towards β-galactosides meaning glycan-based (nano)biosensors lack the required selectivity and affinity. Using a polymer-stabilized nanoparticle biosensing platform, we herein demonstrate that the specificity of immobilised lacto-N-biose towards galectins can be ‘turned on/off’ by using site-specific glycan fluorination and in some cases reversal of specificity can be achieved. The panel of fluoro-glycans were obtained by a chemoenzymatic approach, exploiting BiGalK and BiGalHexNAcP enzymes from Bifidobacterium infantis which are shown to tolerate fluorinated glycans, introducing structural diversity which would be very laborious by chemical methods alone. These results demonstrate that integrating non-natural, fluorinated glycans into nanomaterials can encode unprecedented selectivity with potential applications in biosensing. |
| Document Type: |
article in journal/newspaper |
| File Description: |
text |
| Language: |
English |
| ISSN: |
2041-6520 |
| Relation: |
https://eprints.whiterose.ac.uk/id/eprint/168297/11/d0sc05360k.pdf; Richards, S-J, Keenan, T, Vendeville, J-B et al. (12 more authors) (2021) Introducing affinity and selectivity into galectin-targeting nanoparticles with fluorinated glycan ligands. Chemical Science, 12 (3). pp. 904-910. ISSN: 2041-6520 |
| Availability: |
https://eprints.whiterose.ac.uk/id/eprint/168297/; https://eprints.whiterose.ac.uk/id/eprint/168297/11/d0sc05360k.pdf |
| Rights: |
cc_by_3 |
| Accession Number: |
edsbas.22B4B527 |
| Database: |
BASE |