| Title: |
HLA-E-restricted SARS-CoV-2-specific T cells from convalescent COVID-19 patients suppress virus replication despite HLA class Ia down-regulation |
| Authors: |
Yang, H; Sun, H; Brackenridge, S; Zhuang, X; Wing, PAC; Quastel, M; Walters, L; Garner, L; Wang, B; Yao, X; Felce, SL; Peng, Y; Moore, S; Peeters, BWA; Rei, M; Canto Gomes, J; Tomas, A; Davidson, A; Semple, MG; Turtle, LCW; Openshaw, PJM; Baillie, JK; Mentzer, AJ; Klenerman, P; Investigators, ISARIC4C; Borrow, P; Dong, T; McKeating, JA; Gillespie, GM; McMichael, AJ |
| Publisher Information: |
American Association for the Advancement of Science |
| Publication Year: |
2024 |
| Collection: |
Oxford University Research Archive (ORA) |
| Description: |
Pathogen-specific CD8+ T cell responses restricted by the nonpolymorphic nonclassical class Ib molecule human leukocyte antigen E (HLA-E) are rarely reported in viral infections. The natural HLA-E ligand is a signal peptide derived from classical class Ia HLA molecules that interact with the NKG2/CD94 receptors to regulate natural killer cell functions, but pathogen-derived peptides can also be presented by HLA-E. Here, we describe five peptides from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that elicited HLA-E–restricted CD8+ T cell responses in convalescent patients with coronavirus disease 2019. These T cell responses were identified in the blood at frequencies similar to those reported for classical HLA-Ia–restricted anti–SARS-CoV-2 CD8+ T cells. HLA-E peptide–specific CD8+ T cell clones, which expressed diverse T cell receptors, suppressed SARS-CoV-2 replication in Calu-3 human lung epithelial cells. SARS-CoV-2 infection markedly down-regulated classical HLA class I expression in Calu-3 cells and primary reconstituted human airway epithelial cells, whereas HLA-E expression was not affected, enabling T cell recognition. Thus, HLA-E–restricted T cells could contribute to the control of SARS-CoV-2 infection alongside classical T cells. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
https://doi.org/10.1126/sciimmunol.abl8881 |
| DOI: |
10.1126/sciimmunol.abl8881 |
| Availability: |
https://doi.org/10.1126/sciimmunol.abl8881; https://ora.ox.ac.uk/objects/uuid:bd9d0e30-5500-4516-abad-746760bdab61 |
| Rights: |
info:eu-repo/semantics/openAccess |
| Accession Number: |
edsbas.22F777A0 |
| Database: |
BASE |