| Title: |
Immunomodulatory activity of electrospun polyhydroxyalkanoate fiber scaffolds incorporating olive leaf extract |
| Authors: |
De la Ossa J. G.; Fusco A.; Azimi B.; Salsano J. E.; Digiacomo M.; Coltelli M. B.; De Clerck K.; Roy I.; Macchia M.; Lazzeri A.; Donnarumma G.; Danti S.; Di Stefano R. |
| Contributors: |
De La Ossa, J. G.; Fusco, A.; Azimi, B.; Salsano, J. E.; Digiacomo, M.; Coltelli, M. B.; De Clerck, K.; Roy, I.; Macchia, M.; Lazzeri, A.; Donnarumma, G.; Danti, S.; Di Stefano, R. |
| Publication Year: |
2021 |
| Collection: |
ARPI - Archivio della Ricerca dell'Università di Pisa |
| Subject Terms: |
Anti-inflammatory; Antibacterial; Bio-based; Polyphenol; Tissue regeneration; Wound dressing; Wound healing |
| Description: |
Olive tree is a well-known source of polyphenols. We prepared an olive leaf extract (OLE) and characterized it via high performance liquid chromatography (HPLC) analysis. OLE was blended with different polyhydroxyalkanoates (PHAs), namely, poly(hydroxybutyrate-co-hydroxyvalerate) (PHBHV) and polyhydroxybutyrate/poly(hydroxyoctanoate-co-hydroxydecanoate) (PHB/PHOHD), to produce fiber meshes via electrospinning: OLE/PHBV and OLE/ (PHB/PHOHD), respectively. An 80–90% (w/w%) release of the main polyphenols from the OLE/PHA fibers occurred in 24 h, with a burst release in the first 30 min. OLE and the produced fiber meshes were assayed using human dermal keratinocytes (HaCaT cells) to evaluate the expression of a panel of cytokines involved in the inflammatory process and innate immune response, such as the antimicrobial peptide human beta defensin 2 (HBD-2). Fibers containing OLE were able to decrease the expression of the proinflammatory cytokines at 6 h up to 24 h. All the PHA fibers allowed an early downregulation of the pro-inflammatory cytokines in 6 h, which is suggestive of a strong anti-inflammatory activity exerted by PHA fibers. Differently from pure OLE, PHB/PHOHD fibers (both with and without OLE) upregulated the expression of HBD-2. Our results showed that PHA fiber meshes are suitable in decreasing pro-inflammatory cytokines and the incorporation of OLE may enable indirect antibac-terial properties, which is essential in wound healing and tissue regeneration. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/wos/WOS:000649921000001; volume:11; issue:9; numberofpages:15; journal:APPLIED SCIENCES; https://hdl.handle.net/11568/1108718 |
| DOI: |
10.3390/app11094006 |
| Availability: |
https://hdl.handle.net/11568/1108718; https://doi.org/10.3390/app11094006; https://www.mdpi.com/2076-3417/11/9/4006 |
| Rights: |
info:eu-repo/semantics/openAccess ; license:Creative commons ; license uri:http://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.23316234 |
| Database: |
BASE |