| Title: |
Biological evaluation of a nanostructured vector for osteoarticular pathologies ; Évaluation biologique d’un vecteur nanoparticulaire à visée ostéoarticulaire |
| Authors: |
Riffault, Mathieu |
| Contributors: |
Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA); Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS); Université de Lorraine; Pierre Gillet; Laurent Grossin |
| Source: |
https://hal.univ-lorraine.fr/tel-01751788 ; Médecine humaine et pathologie. Université de Lorraine, 2015. Français. ⟨NNT : 2015LORR0094⟩. |
| Publisher Information: |
CCSD |
| Publication Year: |
2015 |
| Collection: |
Université de Lorraine: HAL |
| Subject Terms: |
Nanoparticles; PLGA; Joint; Hyaluronic acid; Vectorization; Acide polylactique; Nanoparticules; Articulation; Acide hyaluronique; Vectorisation; Médicaments -- Récepteurs; Vecteurs de médicaments; Pharmacologie articulaire; Hyaluronique; Acide; [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| Description: |
One of the major issues with treatment of osteoarticular diseases is reaching cells or tissue inside the joint. Administration of an active compound by intravenous or per os requires elevated amount to be effective, and can initiate systemic and negative side effects. In this work we have evaluated a drug delivery system for hydrophilic molecule, which can be used for articular tissues. Polymeric nanoparticles of poly lactic-co-glycolic acid, labelled with a fluorescent dye were evaluated both in vitro and in vivo. These nanoparticles are covered with hyaluronic acid to favor particles-cells interaction. We demonstrated here that such particles are internalized after 8 hours of exposure in synoviocytes, chondrocytes and mesenchymal stem cells. Evaluation of cytotoxicity and inflammation revealed the neutrality of these particles for articular cell types. Finally, intraarticular injections of particles were performed in healthy rat joint and in pathological models. Histological analyses of extracellular matrix integrity and inflammatory status do not demonstrate any side-effect following particles injections. These healthy nano-device target primarily synovial cells, and their degradation inside cells does not provoke deleterious effects. This PLGA based drug delivery system would be used to safely deliver active molecules to the joint. The combination of these particles with various hydrophilic molecules (as nucleic acid, drugs,…) may allow their direct distribution in the joint, and lead to new therapeutics perspectives. ; Un des écueils du traitement des pathologies ostéoarticulaires est l’adressage de molécules aux cellules et tissus qui constituent l’articulation. L’administration de principes actifs par voie sanguine ou orale nécessite l’apport de quantités importantes et peut causer des effets indésirables. Dans ces travaux, nous avons évalué un système d’adressage de molécules hydrophiles. Des particules de polymère d’acide lactique et glycolique, marquées par un traceur fluorescent ont été évaluées in ... |
| Document Type: |
doctoral or postdoctoral thesis |
| Language: |
French |
| Relation: |
NNT: 2015LORR0094 |
| Availability: |
https://hal.univ-lorraine.fr/tel-01751788; https://hal.univ-lorraine.fr/tel-01751788v1/document; https://hal.univ-lorraine.fr/tel-01751788v1/file/DDOC_T_2015_0094_RIFFAULT.pdf |
| Rights: |
info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.23C0E0F |
| Database: |
BASE |