| Title: |
The Peptide A-3302-B Isolated from a Marine Bacterium Micromonospora sp. Inhibits HSV-2 Infection by Preventing the Viral Egress from Host Cells |
| Authors: |
Sanya Sureram; Irene Arduino; Reiko Ueoka; Massimo Rittà; Rachele Francese; Rattanaporn Srivibool; Dhanushka Darshana; Jörn Piel; Somsak Ruchirawat; Luisa Muratori; David Lembo; Prasat Kittakoop; Manuela Donalisio |
| Source: |
International Journal of Molecular Sciences, Vol 23, Iss 947, p 947 (2022) |
| Publisher Information: |
MDPI AG |
| Publication Year: |
2022 |
| Collection: |
Directory of Open Access Journals: DOAJ Articles |
| Subject Terms: |
rare actinomycete; Micromonospora; marine natural products; natural antiviral products; egress inhibitor; herpes simplex virus type 2; Biology (General); QH301-705.5; Chemistry; QD1-999 |
| Description: |
Herpesviruses are highly prevalent in the human population, and frequent reactivations occur throughout life. Despite antiviral drugs against herpetic infections, the increasing appearance of drug-resistant viral strains and their adverse effects prompt the research of novel antiherpetic drugs for treating lesions. Peptides obtained from natural sources have recently become of particular interest for antiviral therapy applications. In this work, we investigated the antiviral activity of the peptide A-3302-B, isolated from a marine bacterium, Micromonospora sp., strain MAG 9-7, against herpes simplex virus type 1, type 2, and human cytomegalovirus. Results showed that the peptide exerted a specific inhibitory activity against HSV-2 with an EC 50 value of 14 μM. Specific antiviral assays were performed to investigate the mechanism of action of A-3302-B. We demonstrated that the peptide did not affect the expression of viral proteins, but it inhibited the late events of the HSV-2 replicative cycle. In detail, it reduced the cell-to-cell virus spread and the transmission of the extracellular free virus by preventing the egress of HSV-2 progeny from the infected cells. The dual antiviral and previously reported anti-inflammatory activities of A-3302-B, and its effect against an acyclovir-resistant HSV-2 strain are attractive features for developing a therapeutic to reduce the transmission of HSV-2 infections. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
https://www.mdpi.com/1422-0067/23/2/947; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067; https://doaj.org/article/f843ee35ddd64829a957adc455b4fbac |
| DOI: |
10.3390/ijms23020947 |
| Availability: |
https://doi.org/10.3390/ijms23020947; https://doaj.org/article/f843ee35ddd64829a957adc455b4fbac |
| Accession Number: |
edsbas.23F6D0AB |
| Database: |
BASE |