| Title: |
Viral metagenomic analysis of cerebrospinal fluid from patients with acute central nervous system infections of unknown origin, Vietnam |
| Authors: |
Anh, NT; Nhu, LNT; Hong, NTT; Phuc, TM; Tam, PTT; Huong, DT; Anh, TT; Deng, X; Nghia, HDT; Nguyen, TT; Van Hung, N; Thuan, ND; Phuong, PTH; Chau, NVV; Baker, S; Delwart, E; Thwaites, G; Van Tan, L; Consortium1, VIZIONS |
| Publisher Information: |
Centers for Disease Control and Prevention |
| Publication Year: |
2021 |
| Collection: |
Oxford University Research Archive (ORA) |
| Description: |
Central nervous system (CNS) infection is a serious neurologic condition, although the etiology remains unknown in >50% of patients. We used metagenomic next-generation sequencing to detect viruses in 204 cerebrospinal fluid (CSF) samples from patients with acute CNS infection who were enrolled from Vietnam hospitals during 2012–2016. We detected 8 viral species in 107/204 (52.4%) of CSF samples. After virus-specific PCR confirmation, the detection rate was lowered to 30/204 (14.7%). Enteroviruses were the most common viruses detected (n = 23), followed by hepatitis B virus (3), HIV (2), molluscum contagiosum virus (1), and gemycircularvirus (1). Analysis of enterovirus sequences revealed the predominance of echovirus 30 (9). Phylogenetically, the echovirus 30 strains belonged to genogroup V and VIIb. Our results expanded knowledge about the clinical burden of enterovirus in Vietnam and underscore the challenges of identifying a plausible viral pathogen in CSF of patients with CNS infections. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
https://doi.org/10.3201/eid2701.202723 |
| DOI: |
10.3201/eid2701.202723 |
| Availability: |
https://doi.org/10.3201/eid2701.202723; https://ora.ox.ac.uk/objects/uuid:9d0ed3de-5248-4a94-ab8a-c793f63d0bce |
| Rights: |
info:eu-repo/semantics/openAccess ; CC Attribution (CC BY) |
| Accession Number: |
edsbas.2412283D |
| Database: |
BASE |