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The transmembrane protein Syndecan is required for stem cell survival and maintenance of their nuclear properties

Title: The transmembrane protein Syndecan is required for stem cell survival and maintenance of their nuclear properties
Authors: L. Eldridge-Thomas, Buffy; G. Bohere, Jerome; Roubinet, Chantal; Barthelemy, Alexandre; J. Samuels, Tamsin; Teixeira, Felipe, Karam; Kolahgar, Golnar
Contributors: University of Cambridge Cambridge, UK (CAM); Institut de Génétique et Développement de Rennes (IGDR); Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS)-Structure Fédérative de Recherche en Biologie et Santé de Rennes (Biosit : Biologie - Santé - Innovation Technologique); Biologie et génétique du cancer; Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS); Wellcome Trust: 206257/Z/17/Z; Cancer Research UK: 28276; University of Cambridge; Human Frontier Science Program: CDA-00032/2018; Agence Nationale de la Recherche: ANR-20-CE13-0015; HORIZON EUROPE Marie Sklodowska-Curie Actions: 899546; ANR-20-CE13-0015,ACTRICE,Contribution des Jonctions Tricellulaires dans la Mécanique Epithéliale(2020); European Project: 899546,H2020-MSCA-COFUND-2019,H2020-MSCA-COFUND-2019,BIENVENUE(2020)
Source: ISSN: 1553-7390.
Publisher Information: CCSD; Public Library of Science
Publication Year: 2025
Subject Terms: [SDV.GEN]Life Sciences [q-bio]/Genetics
Description: International audience ; Tissue maintenance is underpinned by resident stem cells whose activity is modulated by microenvironmental cues. Using Drosophila as a simple model to identify regulators of stem cell behaviour and survival in vivo, we have identified novel connections between the conserved transmembrane proteoglycan Syndecan, nuclear properties and stem cell function. In the Drosophila midgut, Syndecan depletion in intestinal stem cells results in their loss from the tissue, impairing tissue renewal. At the cellular level, Syndecan depletion alters cell and nuclear shape, and causes nuclear lamina invaginations and DNA damage. In a second tissue, the developing Drosophila brain, live imaging revealed that Syndecan depletion in neural stem cells results in nuclear envelope remodelling defects which arise upon cell division. Our findings reveal a new role for Syndecan in the maintenance of nuclear properties in diverse stem cell types.
Document Type: article in journal/newspaper
Language: English
Relation: info:eu-repo/semantics/altIdentifier/pmid/39913561; info:eu-repo/grantAgreement//899546/EU/Welcoming highly-talented international post-docs in Brittany/BIENVENUE; PUBMED: 39913561
DOI: 10.1371/journal.pgen.1011586
Availability: https://hal.science/hal-04980237; https://hal.science/hal-04980237v1/document; https://hal.science/hal-04980237v1/file/journal.pgen.1011586.pdf; https://doi.org/10.1371/journal.pgen.1011586
Rights: http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess
Accession Number: edsbas.25AC9FA9
Database: BASE