| Title: |
A Fully Integrated Miniaturized Optical Biosensor for Fast and Multiplexing Plasmonic Detection of High- and Low-Molecular-Weight Analytes |
| Authors: |
Bolognesi, Margherita; Prosa, Mario; Toerker, Michael; Lopez Sanchez, Laura; Wieczorek, Martin; Giacomelli, Caterina; Benvenuti, Emilia; Pellacani, Paola; Elferink, Alexander; Morschhauser, Andreas; Sola, Laura; Damin, Francesco; Chiari, Marcella; Whatton, Mark; Haenni, Etienne; Kallweit, David; Marabelli, Franco; Peters, Jeroen; Toffanin, Stefano |
| Source: |
Advanced Materials 35 (2023) 26 ; ISSN: 0935-9648 |
| Publication Year: |
2023 |
| Collection: |
Wageningen UR (University & Research Centre): Digital Library |
| Subject Terms: |
milk safety; on site diagnostics; optical biosensors; organic optoelectronic devices; surface plasmon resonance |
| Description: |
Optical biosensors based on plasmonic sensing schemes combine high sensitivity and selectivity with label-free detection. However, the use of bulky optical components is still hampering the possibility of obtaining miniaturized systems required for analysis in real settings. Here, a fully miniaturized optical biosensor prototype based on plasmonic detection is demonstrated, which enables fast and multiplex sensing of analytes with high- and low molecular weight (80 000 and 582 Da) as quality and safety parameters for milk: a protein (lactoferrin) and an antibiotic (streptomycin). The optical sensor is based on the smart integration of: i) miniaturized organic optoelectronic devices used as light-emitting and light-sensing elements and ii) a functionalized nanostructured plasmonic grating for highly sensitive and specific localized surface plasmon resonance (SPR) detection. The sensor provides quantitative and linear response reaching a limit of detection of 10−4 refractive index units once it is calibrated by standard solutions. Analyte-specific and rapid (15 min long) immunoassay-based detection is demonstrated for both targets. By using a custom algorithm based on principal-component analysis, a linear dose–response curve is constructed which correlates with a limit of detection (LOD) as low as 3.7 µg mL−1 for lactoferrin, thus assessing that the miniaturized optical biosensor is well-aligned with the chosen reference benchtop SPR method. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
https://edepot.wur.nl/633358 |
| DOI: |
10.1002/adma.202208719 |
| Availability: |
https://research.wur.nl/en/publications/a-fully-integrated-miniaturized-optical-biosensor-for-fast-and-mu; https://doi.org/10.1002/adma.202208719; https://edepot.wur.nl/633358 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ ; Wageningen University & Research |
| Accession Number: |
edsbas.26AE3F07 |
| Database: |
BASE |